Protein Info for DDA3937_RS07450 in Dickeya dadantii 3937

Annotation: hydrogenase 4 subunit F

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 558 transmembrane" amino acids 6 to 26 (21 residues), see Phobius details amino acids 34 to 57 (24 residues), see Phobius details amino acids 75 to 95 (21 residues), see Phobius details amino acids 109 to 130 (22 residues), see Phobius details amino acids 136 to 156 (21 residues), see Phobius details amino acids 168 to 188 (21 residues), see Phobius details amino acids 217 to 241 (25 residues), see Phobius details amino acids 255 to 280 (26 residues), see Phobius details amino acids 287 to 306 (20 residues), see Phobius details amino acids 319 to 342 (24 residues), see Phobius details amino acids 348 to 365 (18 residues), see Phobius details amino acids 376 to 397 (22 residues), see Phobius details amino acids 417 to 437 (21 residues), see Phobius details amino acids 457 to 476 (20 residues), see Phobius details PF00662: Proton_antipo_N" amino acids 70 to 101 (32 residues), 29.9 bits, see alignment (E = 4.3e-11) PF00361: Proton_antipo_M" amino acids 132 to 426 (295 residues), 185.4 bits, see alignment E=1.4e-58

Best Hits

Swiss-Prot: 72% identical to HYFF_ECOLI: Hydrogenase-4 component F (hyfF) from Escherichia coli (strain K12)

KEGG orthology group: K12141, hydrogenase-4 component F [EC: 1.-.-.-] (inferred from 100% identity to ddd:Dda3937_00732)

Predicted SEED Role

"Hydrogenase-4 component F"

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.-.-.-

Use Curated BLAST to search for 1.-.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E0SHK8 at UniProt or InterPro

Protein Sequence (558 amino acids)

>DDA3937_RS07450 hydrogenase 4 subunit F (Dickeya dadantii 3937)
MTTLDLFSLLLGVPFVAALLAFACRFTGTAARALVSLIHLLGISALLLVALLAVWTVYQQ
GELLAAHRWLHLDSLSALFLAILGVIGFLTGLYSMGYMRHEVDGGEISVTTLCHYYGFFH
LFLFTMLLVITSNNLILMWAAIEATTLSSAFLVGLYGQRSSLEAAWKYIIICTVGVAFGL
YGTVLVYANAANVMAEPGNAIFWTEVLQHAGELDSTLMHLAFIFILIGFGTKTGLFPMHA
WLPDAHSEAPSPTSALLSAVLLNCALLVIVRYTILISAAIGPEFPQRLLLVFGLLSVAVA
AFLILVQRDMKRLLAYSSVENMGLIAVALGIGGPLGILAALLHTLNHSLAKTLLFCGSGN
VLLKYGTRDMDAVKGILRVAPVTGALLAGGALALGGMPPFNVFLSEFMTVTAGIHAGHLP
LVLVLLVLLTVVLAGLVRMIATSVLGSQPEAVSKGELGWLTTAPMLILLVLMLVMGTRIP
QPVIHLLEQATAIVLNGNHAGEPTPPSLGWPSLSARTTTLGAATRGTTTMSTTTLGTTSP
DTAPASSLTPSRQEMYRD