Protein Info for DDA3937_RS07405 in Dickeya dadantii 3937

Annotation: formate dehydrogenase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 716 PF04879: Molybdop_Fe4S4" amino acids 1 to 53 (53 residues), 56.7 bits, see alignment 2.9e-19 TIGR01591: formate dehydrogenase, alpha subunit" amino acids 6 to 683 (678 residues), 929.7 bits, see alignment E=4.1e-284 PF00384: Molybdopterin" amino acids 61 to 484 (424 residues), 283.9 bits, see alignment E=2.8e-88 PF01568: Molydop_binding" amino acids 574 to 681 (108 residues), 104.5 bits, see alignment E=4.8e-34

Best Hits

Swiss-Prot: 67% identical to FDHF_ECOLI: Formate dehydrogenase H (fdhF) from Escherichia coli (strain K12)

KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 100% identity to ddd:Dda3937_00742)

MetaCyc: 67% identical to formate dehydrogenase H (Escherichia coli K-12 substr. MG1655)
RXN0-3281 [EC: 1.17.98.4]; FHLMULTI-RXN [EC: 1.17.98.4]

Predicted SEED Role

"Formate dehydrogenase H (EC 1.2.1.2) @ selenocysteine-containing" (EC 1.2.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.17.98.4 or 1.2.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E0SHJ8 at UniProt or InterPro

Protein Sequence (716 amino acids)

>DDA3937_RS07405 formate dehydrogenase subunit alpha (Dickeya dadantii 3937)
MQKVITVCPYCGSGCKINLLVENGKVVGAEGANGLTNEGELCLKGYYGWDFLNDTKLLTP
RLKQPMIRRQKGAPFEVVSWDEAIDFASSRLKAIKEKYGPDAIMHTGSSRGPGNETNYVM
QKFARAVTGTNNIDCCARVCHGPSVSGLQVTLGNGAMSNSICEIEHTDCILVFGYNAADS
HPIVARRIIKAKERGAKIIVCDPRHIETARIADLWLPLKNGSNMALVNAFANVLINEGLY
NANFVAQHTEGFEEYRNLVAKYTPEYVADITGLDPQLIRDAIRMYAAAPSATILWGMGVT
QWTQGVDVVKGLSGLALLTGNLGRPNVGVGPVRGQNNVQGACDMGALPNQFPGYQQVTDA
DVREKFAKAWGVPSLSDRIGYSLTDVPHMIKEGKIKANYLMGEDPLQTEPDLSVVRETFN
QLELLIVQDIFMTKTAAVADVIFPATSWGEHEGVYSAADRGFQRFYKAVEPQGNVKPDWE
IISLMATALGYPMHYNNTHEIWDELRNLCPLYYGATYEKMAGLGYVPWPCLTEDSPGTPW
LYAGNQFDRPNGKGLLFATEWHPPMEQTDADYPLVLSTVREVGHYSCRSMTGNCTALQTL
ADEPGYVQMNPEDAARLGIRDQQLTWVASRRGKVISRAMVSERINKGAVYMTYQWWIGAC
NELTLDEVDPIAKTPEYKHCAVKLEPIADQNWAENYVVQEYSALKARLRKEAEVAG