Protein Info for DDA3937_RS07140 in Dickeya dadantii 3937

Annotation: glycine betaine/L-proline transporter ProP

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 501 transmembrane" amino acids 36 to 57 (22 residues), see Phobius details amino acids 65 to 87 (23 residues), see Phobius details amino acids 99 to 117 (19 residues), see Phobius details amino acids 124 to 147 (24 residues), see Phobius details amino acids 168 to 191 (24 residues), see Phobius details amino acids 199 to 219 (21 residues), see Phobius details amino acids 261 to 279 (19 residues), see Phobius details amino acids 299 to 319 (21 residues), see Phobius details amino acids 328 to 346 (19 residues), see Phobius details amino acids 352 to 377 (26 residues), see Phobius details amino acids 389 to 412 (24 residues), see Phobius details amino acids 418 to 439 (22 residues), see Phobius details PF00083: Sugar_tr" amino acids 29 to 450 (422 residues), 246.3 bits, see alignment E=9.3e-77 TIGR00883: MFS transporter, metabolite:H+ symporter (MHS) family protein" amino acids 34 to 433 (400 residues), 493 bits, see alignment E=3.6e-152 PF07690: MFS_1" amino acids 62 to 311 (250 residues), 83.9 bits, see alignment E=1.6e-27 amino acids 276 to 451 (176 residues), 54.3 bits, see alignment E=1.6e-18 PF08946: Osmo_CC" amino acids 455 to 500 (46 residues), 75.3 bits, see alignment 5.5e-25

Best Hits

Swiss-Prot: 100% identical to OUSA_DICD3: Glycine betaine/proline/ectoine/pipecolic acid transporter OusA (ousA) from Dickeya dadantii (strain 3937)

KEGG orthology group: K03762, MFS transporter, MHS family, proline/betaine transporter (inferred from 100% identity to ddd:Dda3937_00791)

MetaCyc: 85% identical to osmolyte:H+ symporter ProP (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-29; TRANS-RXN-29A

Predicted SEED Role

"L-Proline/Glycine betaine transporter ProP" in subsystem Proline, 4-hydroxyproline uptake and utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q47421 at UniProt or InterPro

Protein Sequence (501 amino acids)

>DDA3937_RS07140 glycine betaine/L-proline transporter ProP (Dickeya dadantii 3937)
MKLKRKRVKPIALDDVTIIDDGRLRKAITAAALGNAMEWFDFGVYGFVAYALGQVFFPGA
DPGVQMIAALATFSVPFLIRPLGGVFFGALGDKYGRQKILAITIIIMSISTFCIGLIPSY
ERIGIWAPILLLLAKMAQGFSVGGEYTGASIFVAEYSPDRKRGFMGSWLDFGSIAGFVLG
AGVVVLISTLIGEQAFLAWGWRLPFFLALPLGLIGLYLRHALEETPAFRQHVEKLEQNDR
DGLKAGPGVSFREIATHHWKSLLVCIGLVIATNVTYYMLLTYMPSYLSHSLHYSENHGVL
IIIAIMIGMLFVQPVMGLLSDRFGRKPFVVIGSVAMFFLAVPSFMLINSDIIGLIFLGLL
MLAVILNAFTGVMASTLPALFPTHIRYSALASAFNISVLIAGLTPTVAAWLVESSQNLYM
PAYYLMVIAVIGLLTGLFMKETANKPLKGATPAASDLSEAKEILQEHHDNIEHKIEDITQ
QIAELEAKRQLLVAQHPRIND