Protein Info for DDA3937_RS05645 in Dickeya dadantii 3937

Annotation: ATP-dependent protease ATP-binding subunit ClpX

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 424 TIGR00382: ATP-dependent Clp protease, ATP-binding subunit ClpX" amino acids 5 to 412 (408 residues), 701 bits, see alignment E=2.6e-215 PF06689: zf-C4_ClpX" amino acids 14 to 50 (37 residues), 72.8 bits, see alignment 6.6e-24 PF00493: MCM" amino acids 69 to 193 (125 residues), 25.4 bits, see alignment E=2.9e-09 PF07724: AAA_2" amino acids 112 to 310 (199 residues), 115.2 bits, see alignment E=1.3e-36 PF07728: AAA_5" amino acids 114 to 191 (78 residues), 25.2 bits, see alignment E=5.9e-09 PF00004: AAA" amino acids 115 to 219 (105 residues), 57 bits, see alignment E=1.2e-18 PF10431: ClpB_D2-small" amino acids 317 to 393 (77 residues), 45.4 bits, see alignment E=2.6e-15

Best Hits

Swiss-Prot: 96% identical to CLPX_PECCP: ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) from Pectobacterium carotovorum subsp. carotovorum (strain PC1)

KEGG orthology group: K03544, ATP-dependent Clp protease ATP-binding subunit ClpX (inferred from 100% identity to ddd:Dda3937_03318)

MetaCyc: 92% identical to ATP-dependent Clp protease ATP-binding subunit ClpX (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"ATP-dependent Clp protease ATP-binding subunit ClpX" in subsystem Proteasome bacterial or Proteolysis in bacteria, ATP-dependent

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E0SD70 at UniProt or InterPro

Protein Sequence (424 amino acids)

>DDA3937_RS05645 ATP-dependent protease ATP-binding subunit ClpX (Dickeya dadantii 3937)
MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLCNDIIREEIKEVAPHRE
RSALPTPHEIRRHLDDYVIGQEQAKKVLAVAVYNHYKRLRNGDSNNSIELGKSNILLIGP
TGSGKTLLAETLARFLDVPFTMADATTLTEAGYVGEDVENIIQKLLQKCDYDVQKAQRGI
VYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGTIAAVPPQGGRKHPQQEFLQVDT
SKILFICGGAFAGLDKVIEQRTDTGRGIGFNATVKGLSQKATEGELLSQVEPGDLIKFGL
IPEFIGRLPVVATLKELDEEALIQILREPKNALTKQYQALFKLEGAELEFRDEALTAIAK
KAMARKTGARGLRSIVEAALLETMYDLPSLENVDKVVIDDSVISGQTEPLLIYGKPEAQQ
ASGE