Protein Info for DDA3937_RS05085 in Dickeya dadantii 3937

Annotation: tRNA pseudouridine(65) synthase TruC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 261 PF00849: PseudoU_synth_2" amino acids 10 to 170 (161 residues), 121.6 bits, see alignment E=1.7e-39

Best Hits

Swiss-Prot: 76% identical to TRUC_YERPE: tRNA pseudouridine synthase C (truC) from Yersinia pestis

KEGG orthology group: K06175, tRNA pseudouridine synthase C [EC: 5.4.99.12] (inferred from 100% identity to ddd:Dda3937_00873)

MetaCyc: 73% identical to tRNA pseudouridine65 synthase (Escherichia coli K-12 substr. MG1655)
RXN-11840 [EC: 5.4.99.26]

Predicted SEED Role

"tRNA pseudouridine synthase C (EC 4.2.1.70)" (EC 4.2.1.70)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12 or 5.4.99.26

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E0SCC3 at UniProt or InterPro

Protein Sequence (261 amino acids)

>DDA3937_RS05085 tRNA pseudouridine(65) synthase TruC (Dickeya dadantii 3937)
MLEIVYQDEHLIAVNKPAGWLVHRSWLDRKEKVVVMQTVRDQIGQHVFTVHRLDRPTSGV
LLMALSPEVARLLAQQFEQHQMEKTYHAVVRGYVMEDGVIDYALTEELDRIADKFADPDK
APQPAVSHYRVLARAEMPVAIGRYDTARYSLLQLNPKTGRKHQLRRHMAHLRHPIIGDTT
HGDLRQNRGMAAHFDCARLMLHASCLSLTHPVTNEPLAISARWDEPWQGVMTKFGWQGSL
PDVERVEFPLASGQDNDLTSA