Protein Info for DDA3937_RS04950 in Dickeya dadantii 3937

Annotation: phosphoenolpyruvate--protein phosphotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 748 transmembrane" amino acids 144 to 157 (14 residues), see Phobius details PF13185: GAF_2" amino acids 17 to 154 (138 residues), 58.1 bits, see alignment E=3.6e-19 PF01590: GAF" amino acids 18 to 153 (136 residues), 70.1 bits, see alignment E=8.6e-23 PF13492: GAF_3" amino acids 19 to 151 (133 residues), 35.5 bits, see alignment E=3.6e-12 PF05524: PEP-utilisers_N" amino acids 173 to 294 (122 residues), 78.2 bits, see alignment E=1.8e-25 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 176 to 730 (555 residues), 410.8 bits, see alignment E=4.3e-127 PF00391: PEP-utilizers" amino acids 320 to 391 (72 residues), 52.9 bits, see alignment E=7.4e-18 PF02896: PEP-utilizers_C" amino acids 420 to 706 (287 residues), 349.9 bits, see alignment E=3.2e-108

Best Hits

Swiss-Prot: 73% identical to PT1P_SALTY: Phosphoenolpyruvate-dependent phosphotransferase system (ptsP) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K08484, phosphotransferase system, enzyme I, PtsP [EC: 2.7.3.9] (inferred from 100% identity to ddd:Dda3937_00849)

MetaCyc: 73% identical to phosphoenolpyruvate-protein phosphotransferase PtsP (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Phosphoenolpyruvate-protein phosphotransferase, nitrogen regulation associated"

Isozymes

Compare fitness of predicted isozymes for: 2.7.3.9

Use Curated BLAST to search for 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E0SBV0 at UniProt or InterPro

Protein Sequence (748 amino acids)

>DDA3937_RS04950 phosphoenolpyruvate--protein phosphotransferase (Dickeya dadantii 3937)
MLTRLREIIEKVAAAARLSDALDILVNETCLAMDTEVCSIYLADHDRQCYYLMATRGLKK
PRGRTITLAFGQGIVGLVGERAEPINLADAQSHPSFKFIPAVREQHFRSFLGVPVIHRRQ
LLGVLVVQQREHRQFDKNEESFMVTLATQMAAILSLSQMKALFGQYRQTRIKAMAASSGV
AIAIGWQDRGQPSLELVFPASSLDSDRERSRLLMAMEEAGAEFRRFSKRFSASAQKESAA
IFDFYSHLLNDARLKRELFQEVDTGSVAEWAVKVVIERFAAQFASLQDPYLRDRSSDLRA
LGQRLLFHLDDNAQSNGQWPERFILVADELTATLLAEVPQERLAGVVAYDGAANSHAAIL
VRAMGIPTLMGADIQPELLHQRLLVLDGYRGELLVDPEPVLVQEYQRLLSEENELTRLAE
DDMERPAVLKSGERVEVMLNAGLSAEHEKRFINQVDGVGLYRTEIPFMLQNGFPSEEEQM
TQYEGMLRLYPSRPVMLRTLDIGADKQLPYLPISEENPCLGWRGIRVTLDQPEIFMIQVR
AMLRANAHIGNLSILLPMINSLDEIDEAKRLIDRAAAEVAEMLGFPQPRPRIGIMIEVPS
VLFLLPHLASRIDFVSVGTNDLTQYLLAVDRNNPHVGALYDSLHPSMLQALNMIITHCRQ
YSLPVSVCGEMAGEPMGALLLIGLGYRTLSMNGRSVARIKYLLRRIGEDEARQLAGKVLQ
AQTASEVRQWASIFIEERGLGGLIRGGR