Protein Info for DDA3937_RS02750 in Dickeya dadantii 3937
Annotation: glycerol-3-phosphate 1-O-acyltransferase PlsB
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 83% identical to PLSB_PECCP: Glycerol-3-phosphate acyltransferase (plsB) from Pectobacterium carotovorum subsp. carotovorum (strain PC1)
KEGG orthology group: K00631, glycerol-3-phosphate O-acyltransferase [EC: 2.3.1.15] (inferred from 100% identity to ddd:Dda3937_02724)MetaCyc: 77% identical to glycerol-3-phosphate 1-O-acyltransferase (Escherichia coli K-12 substr. MG1655)
Glycerol-3-phosphate O-acyltransferase. [EC: 2.3.1.15]; 2.3.1.15 [EC: 2.3.1.15]; 2.3.1.15 [EC: 2.3.1.15]; 2.3.1.15 [EC: 2.3.1.15]; 2.3.1.15 [EC: 2.3.1.15]
Predicted SEED Role
"Glycerol-3-phosphate acyltransferase (EC 2.3.1.15)" in subsystem Glycerolipid and Glycerophospholipid Metabolism in Bacteria (EC 2.3.1.15)
MetaCyc Pathways
- superpathway of phospholipid biosynthesis III (E. coli) (11/12 steps found)
- phosphatidylglycerol biosynthesis I (6/6 steps found)
- phosphatidylglycerol biosynthesis II (6/6 steps found)
- CDP-diacylglycerol biosynthesis I (4/4 steps found)
- CDP-diacylglycerol biosynthesis II (4/4 steps found)
- superpathway of cardiolipin biosynthesis (bacteria) (10/13 steps found)
- oleate biosynthesis III (cyanobacteria) (2/3 steps found)
- phosphatidate biosynthesis (yeast) (3/5 steps found)
- diacylglycerol and triacylglycerol biosynthesis (3/7 steps found)
- palmitoyl ethanolamide biosynthesis (2/6 steps found)
- superpathway of stearidonate biosynthesis (cyanobacteria) (2/6 steps found)
- stigma estolide biosynthesis (2/7 steps found)
- anandamide biosynthesis II (2/8 steps found)
- anandamide biosynthesis I (4/12 steps found)
- superpathway of phospholipid biosynthesis II (plants) (10/28 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.3.1.15
Use Curated BLAST to search for 2.3.1.15
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See E0SK36 at UniProt or InterPro
Protein Sequence (829 amino acids)
>DDA3937_RS02750 glycerol-3-phosphate 1-O-acyltransferase PlsB (Dickeya dadantii 3937) MSGWRRIYYKLLNFPLKLLVKSKVIPQDPVNEQRLDPARPIFYVLPYNSKADLLTLRTKC LELSLPDPLQPLQIDGKILPSYVFVNDGPRLFRYYVPKQQSIKLFHDYLDLHRNNPELDI QMVPVSVMFGRSPGREGQNQDTPHLRLLNGIEKFFAVLWLGRDSFVRFSVPVSLRYMATE HGTDKTIAHKLARVARMHFSRLRLAAVGPRLPVRQELFNKLLSSRAIAKAIDDEARVKKI SHDKAQQNAITLLEEIAADFSYEAVRLSDRVLSWTWNRLYQGINVHNAERVRQLAQDGHE IVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGINLNFWPAGPIFRRLGAFFIRRTFKGN KLYSTLFREYLGELFTRGYSVEYFVEGGRSRTGRLLEPKTGTLAMTIQAMLRGGTRQITL VPIYIGYEHVMEVGTYAKELRGATKEKEGFLQMVRGLRKLRNLGQGYVNIGEPLSLTTYL NNRVPQWRDAIDPIDPQRPAWLTPTVQDIADDIMVRINNAAAANAMNLCCTALLASRQRA LTREQMEEQLECYLQLLRNVPYSSDITVPKKTATELLEHALGMDKFEVEKDNVGDIIILP REQAVLMTYYRNNIHHMLVLPSLVASIVMHYRRISAGELLRQITLVYPLLQAELFLHYKQ SELSDVLDALVAELARQQLILIKDDGLELNPARIRALQLLAAGVRETLQRYAITLALLNA NPTISRGALEKESRSMAQRLSVLHGINAPEFFDKAVFSTLVNTLRNEGYVSDSGEAVLVH VQAIYAILGELLSPEVKLTIESASLAQESGDGDSESVVGDVEKEKEEVE