Protein Info for DDA3937_RS02545 in Dickeya dadantii 3937

Annotation: heavy metal translocating P-type ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 812 transmembrane" amino acids 184 to 203 (20 residues), see Phobius details amino acids 209 to 227 (19 residues), see Phobius details amino acids 239 to 255 (17 residues), see Phobius details amino acids 261 to 277 (17 residues), see Phobius details amino acids 412 to 431 (20 residues), see Phobius details amino acids 443 to 469 (27 residues), see Phobius details amino acids 748 to 769 (22 residues), see Phobius details amino acids 775 to 796 (22 residues), see Phobius details TIGR01511: copper-translocating P-type ATPase" amino acids 224 to 793 (570 residues), 397.4 bits, see alignment E=2.5e-122 TIGR01525: heavy metal translocating P-type ATPase" amino acids 241 to 795 (555 residues), 590.3 bits, see alignment E=9.3e-181 TIGR01494: HAD ATPase, P-type, family IC" amino acids 264 to 771 (508 residues), 252.1 bits, see alignment E=1.7e-78 PF00122: E1-E2_ATPase" amino acids 292 to 473 (182 residues), 192.7 bits, see alignment E=6.3e-61 PF00702: Hydrolase" amino acids 492 to 706 (215 residues), 110.6 bits, see alignment E=2.1e-35

Best Hits

KEGG orthology group: K01534, Cd2+/Zn2+-exporting ATPase [EC: 3.6.3.3 3.6.3.5] (inferred from 100% identity to ddd:Dda3937_02650)

MetaCyc: 62% identical to cadmium translocating P-type ATPase (Pseudomonas putida KT2440)
3.6.3.3-RXN [EC: 7.2.2.21]

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5 or 7.2.2.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E0SJZ4 at UniProt or InterPro

Protein Sequence (812 amino acids)

>DDA3937_RS02545 heavy metal translocating P-type ATPase (Dickeya dadantii 3937)
MIPLDPVVATGFLLQQNPEQESTMSEKHSPTSRRQFQPVALEKPGKALFRPHQQGHAGLG
QTAPNSALAQHSEQSCANHCCDNSAPPQPTSELTSAERTGDQQRTPIRIMQMDCPTEEAM
LRKKLDALPEVSELEFNLMQRVMTVTHRYDALDKVLTAIRSLGFEPEVRTENDRTPLPPE
QKKAWWPLGLAVVAAAAAEAVEWTGLPEWWAAALAILAVAASGLTTYRKGWIALRTGNLN
INTLMSIAVTGALFLQQWPEAAMVMVLFTLAEHIEARSLDRARNAIAGLMNLAPDTATVQ
QPDGDWREVEAKTVTPGSIVRVRPGERIALDGEITSGHSAVNQAPITGESLPVDKRVGDS
VFAGTINESGSFEYRVTAAAANTTLARIIHAVEQAQGTKAPTQRFVDQFARVYTPLVFLG
ALLVAVLPPLFADGDWLDWTYKALVLLVIACPCALVISTPVTIVSGLAAAARRGILIKGG
VFLEKGHALAWLALDKTGTLTHGKPVQTGFESVADVDDARCRQLAASLASRSDHPVSQAV
ARAAQQAGTPLIDVDDFSAVAGQGVIGTLQGQRYFLGNQRLARLWLGDAADGIIERLSAL
EQAGNTAIILGDGRQILALMAVADTVKPSSQEAINALHQAGIKTLMLTGDNQHVAQAIAR
DVGIDEARGNLLPEDKLSQIERLSAQGVTGMVGDGINDTPALARADIGFAMGAMGTDSAI
ETADVALMNDDLRKIPEFVRISKATRAILIQNIVLALGIKALFLTLTLLGMGTMWMAVFA
DVGASLLVVGNGLRLLRQAPVSTRQHQQPQPA