Protein Info for DDA3937_RS01915 in Dickeya dadantii 3937

Annotation: 2-iminobutanoate/2-iminopropanoate deaminase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 90% identical to RIDA_SALTY: 2-iminobutanoate/2-iminopropanoate deaminase (ridA) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K09022, UPF0076 protein YjgF (inferred from 99% identity to ddc:Dd586_0372)

MetaCyc: 90% identical to 2-iminobutanoate/2-iminopropanoate deaminase monomer (Salmonella enterica enterica serovar Typhimurium str. LT2)
Tryptophanase. [EC: 3.5.99.10, 4.1.99.1, 4.1.99.2, 4.3.1.13, 4.3.1.17, 4.3.1.18, 4.3.3.8, 4.4.1.1, 4.4.1.13, 4.4.1.15, 4.4.1.25, 4.4.1.28, 4.4.1.35, 4.5.1.2]; 3.5.99.10,4.3.1.-,4.3.1.19,4.4.1.1,4.4.1.1,4.4.1.2,3.5.99.7,4.4.1.11 [EC: 3.5.99.10, 4.1.99.1, 4.1.99.2, 4.3.1.13, 4.3.1.17, 4.3.1.18, 4.3.3.8, 4.4.1.1, 4.4.1.13, 4.4.1.15, 4.4.1.25, 4.4.1.28, 4.4.1.35, 4.5.1.2, 3.5.99.7, 4.3.1.19, 4.4.1.11, 4.4.1.2]

Predicted SEED Role

"Bona fide RidA/YjgF/TdcF/RutC subgroup"

MetaCyc Pathways

aspartate superpathway (24/25 steps found)
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (18/18 steps found)
superpathway of branched chain amino acid biosynthesis (17/17 steps found)
superpathway of L-isoleucine biosynthesis I (13/13 steps found)
superpathway of S-adenosyl-L-methionine biosynthesis (9/9 steps found)
superpathway of L-methionine biosynthesis (transsulfuration) (9/9 steps found)
superpathway of L-homoserine and L-methionine biosynthesis (8/8 steps found)
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
L-threonine degradation I (6/6 steps found)
L-methionine biosynthesis I (5/5 steps found)
superpathway of L-threonine metabolism (14/18 steps found)
D-serine degradation (3/3 steps found)
L-serine degradation (3/3 steps found)
L-tryptophan degradation II (via pyruvate) (3/3 steps found)
glycine degradation (3/3 steps found)
L-methionine biosynthesis II (5/6 steps found)
L-cysteine degradation II (2/3 steps found)
L-methionine degradation II (2/3 steps found)
sulfolactate degradation III (2/3 steps found)
superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (7/10 steps found)
L-threonine degradation V (1/2 steps found)
seleno-amino acid biosynthesis (plants) (3/5 steps found)
homocysteine and cysteine interconversion (2/4 steps found)
L-cysteine biosynthesis VI (reverse transsulfuration) (4/7 steps found)
glycine betaine degradation III (4/7 steps found)
superpathway of L-cysteine biosynthesis (fungi) (3/6 steps found)
superpathway of sulfolactate degradation (3/6 steps found)
felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis (2/5 steps found)
L-mimosine degradation (4/8 steps found)
glycine betaine degradation I (4/8 steps found)
superpathway of L-cysteine biosynthesis (mammalian) (1/5 steps found)
glutathione-mediated detoxification I (3/8 steps found)
hydrogen sulfide biosynthesis II (mammalian) (1/6 steps found)
purine nucleobases degradation II (anaerobic) (13/24 steps found)
glutathione-mediated detoxification II (1/9 steps found)
hypoglycin biosynthesis (4/14 steps found)
superpathway of L-methionine salvage and degradation (5/16 steps found)
superpathway of seleno-compound metabolism (6/19 steps found)
cyclosporin A biosynthesis (2/15 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.99.7, 4.1.99.1, 4.3.1.17, 4.3.1.18, 4.3.1.19, 4.4.1.15

Use Curated BLAST to search for 3.5.99.10 or 3.5.99.7 or 4.1.99.1 or 4.1.99.2 or 4.3.1.13 or 4.3.1.17 or 4.3.1.18 or 4.3.1.19 or 4.3.3.8 or 4.4.1.1 or 4.4.1.11 or 4.4.1.13 or 4.4.1.15 or 4.4.1.2 or 4.4.1.25 or 4.4.1.28 or 4.4.1.35 or 4.5.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E0SI88 at UniProt or InterPro

Protein Sequence (128 amino acids)

>DDA3937_RS01915 2-iminobutanoate/2-iminopropanoate deaminase (Dickeya dadantii 3937)
MSRIISTEQAPAAIGPYVQGVDLGSMIITSGQIPVDPKTGLVPDDVTAQARQSLENVKAI
VEAAGLKVSNIVKTTVFVKDLNDFATVNAAYEAFFTEHNAPFPARSCVEVARLPKDVKIE
IEAIAVRG