Protein Info for DDA3937_RS01220 in Dickeya dadantii 3937

Annotation: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00355: phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase
  • amino acids 8 to 529 (522 residues), 878.5 bits, E=8e-269
• PF02142: MGS
  • amino acids 19 to 132 (114 residues), 92.7 bits, E=1.3e-30
• PF01808: AICARFT_IMPCHas
  • amino acids 137 to 459 (323 residues), 428.7 bits, E=1.5e-132

Best Hits

Swiss-Prot: 92% identical to PUR9_PECAS: Bifunctional purine biosynthesis protein PurH (purH) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)

KEGG orthology group: K00602, phosphoribosylaminoimidazolecarboxamide formyltransferase / IMP cyclohydrolase [EC: 2.1.2.3 3.5.4.10] (inferred from 100% identity to ddd:Dda3937_00244)

MetaCyc: 90% identical to bifunctional AICAR transformylase/IMP cyclohydrolase (Escherichia coli K-12 substr. MG1655)
IMP cyclohydrolase. [EC: 3.5.4.10]; Phosphoribosylaminoimidazolecarboxamide formyltransferase. [EC: 3.5.4.10, 2.1.2.3]

Predicted SEED Role

"IMP cyclohydrolase (EC 3.5.4.10) / Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC 2.1.2.3)" in subsystem De Novo Purine Biosynthesis (EC 2.1.2.3, EC 3.5.4.10)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (46/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (26/26 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• inosine-5'-phosphate biosynthesis I (6/6 steps found)
• inosine-5'-phosphate biosynthesis II (5/5 steps found)
• inosine-5'-phosphate biosynthesis III (5/6 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of plant hormones
• One carbon pool by folate
• Purine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.2.3 or 3.5.4.10

Sequence Analysis Tools

See E0SGH8 at UniProt or InterPro

Protein Sequence (529 amino acids)

>DDA3937_RS01220 bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase (Dickeya dadantii 3937)
MQQRRPIRRALLSVSDKAGIVEFAQALSQRGIDLLSTGGTARLLADAGLPVTEVSDYTGF
PEMMDGRVKTLHPKVHGGILGRRGQDDAIMTQHNIQPIDMVVVNLYPFAQTVAKADCTLE
DAVENIDIGGPTMVRSAAKNHNDVAIVVKSSDYTAIITEMDANEGSLTYETRFDLAIKAF
EHTAAYDSMIANYFGSKVPAYHGDTTQPSGHFPRTLNLNFIKKQDMRYGENSHQQAAFYI
EEKVAEASVATAQQLQGKALSYNNIADTDAALECVKEFVEPACVIVKHANPCGVAIGDSI
LDAYERAYKTDPTSAFGGIIAFNRELDEATAQAIISRQFVEVIIAPSASEAALKVTAAKQ
NVRVLTCGQWQQRVSGLDFKRVNGGLLVQDRDLGMVEAAQLRVVTERQPTEAELRDALFC
WKVAKFVKSNAIVYARDNMTIGIGAGQMSRVYSAKIAGIKAGDEGLEVKGSVMASDAFFP
FRDGIDAAAAVGITCVIQPGGSIRDDEVIAAANEHGIAMLFTDMRHFRH