Protein Info for DDA3937_RS01155 in Dickeya dadantii 3937

Annotation: thiazole synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 83% identical to THIG_PECAS: Thiazole synthase (thiG) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)

KEGG orthology group: K03149, thiamine biosynthesis ThiG (inferred from 100% identity to ddd:Dda3937_00231)

MetaCyc: 77% identical to 1-deoxy-D-xylulose 5-phosphate:thiol sulfurtransferase (Escherichia coli K-12 substr. MG1655)
THIAZOLSYN2-RXN [EC: 2.8.1.10]

Predicted SEED Role

"Thiazole biosynthesis protein ThiG" in subsystem Thiamin biosynthesis

MetaCyc Pathways

superpathway of thiamine diphosphate biosynthesis I (10/10 steps found)
thiazole component of thiamine diphosphate biosynthesis I (6/6 steps found)
superpathway of thiamine diphosphate biosynthesis II (9/11 steps found)
thiazole component of thiamine diphosphate biosynthesis II (5/7 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.1.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E0SGG5 at UniProt or InterPro

Protein Sequence (259 amino acids)

>DDA3937_RS01155 thiazole synthase (Dickeya dadantii 3937)
MLKIADITFTSRLLTGTGKFSSAKLMQDALHASGSQLVTLAMKRVDLKSGSDAILAPLRE
LGVHLLPNTSGAKTAEEAVFAARLAREALGTHWVKLEIHPDMKYLLPDPVETLKAAEQLV
KDGFVVLPYCGADPVLCKRLEDAGCAAVMPLGAPIGSNQGLQTRDFLRIIIEQARVPVVV
DAGIGAPSHAAEAIELGADAVLVNTAIAVARDPVQMAHAFRLAVDAGAIARQAGLGNRQR
IASATSPLTGFLQHTEVAS