Protein Info for DDA3937_RS01150 in Dickeya dadantii 3937

Annotation: 2-iminoacetate synthase ThiH

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 387 TIGR02351: thiazole biosynthesis protein ThiH" amino acids 15 to 378 (364 residues), 532.9 bits, see alignment E=1.9e-164 PF04055: Radical_SAM" amino acids 92 to 242 (151 residues), 48.2 bits, see alignment E=1.5e-16 PF06968: BATS" amino acids 269 to 371 (103 residues), 52.7 bits, see alignment E=4.1e-18

Best Hits

Swiss-Prot: 80% identical to THIH_SALTY: 2-iminoacetate synthase (thiH) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K03150, thiamine biosynthesis ThiH (inferred from 100% identity to ddd:Dda3937_00230)

MetaCyc: 81% identical to 2-iminoacetate synthase (Escherichia coli K-12 substr. MG1655)
RXN-11319 [EC: 4.1.99.19]

Predicted SEED Role

"2-iminoacetate synthase (ThiH) (EC 4.1.99.19)" (EC 4.1.99.19)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.99.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E0SGG4 at UniProt or InterPro

Protein Sequence (387 amino acids)

>DDA3937_RS01150 2-iminoacetate synthase ThiH (Dickeya dadantii 3937)
MDSQTIDGKHQPPSFERHWQQLEWHDLTLRINSKTDADVERALCADRLTREDMMALLSPA
AGRWLEPLAQRAQQLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNHLKRKTLDEQE
ILRECEAIKALGFDHLLLVTGEHQRKVGMDYFRQMLPLIRPQFSSLMMEVQPLSQAEYAE
LKTLGLDGVMVYQETYHPATYARHHLRGHKQDFAWRLATPDRLGRAGIDKIGLGALIGLS
DSWRTDCYMVAEHLLYLQQTYWQSRYSVSFPRLRPCAGGIEPASLMDEAQLMQVICAFRL
LAPDVELSLSTRESPFFRDHMIPIAINNVSAFSKTQPGGYADDHPELEQFTPHDNRRPQA
VADALTRAGLQPVWKDWDGYLGRPVTG