Protein Info for DDA3937_RS01030 in Dickeya dadantii 3937

Annotation: bifunctional biotin--[acetyl-CoA-carboxylase] ligase/biotin operon repressor BirA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 319 transmembrane" amino acids 123 to 137 (15 residues), see Phobius details amino acids 145 to 164 (20 residues), see Phobius details TIGR00122: biotin operon repressor" amino acids 6 to 74 (69 residues), 83.9 bits, see alignment E=5.8e-28 PF08279: HTH_11" amino acids 9 to 58 (50 residues), 37 bits, see alignment 3.7e-13 TIGR00121: biotin--[acetyl-CoA-carboxylase] ligase" amino acids 81 to 317 (237 residues), 252.3 bits, see alignment E=4.4e-79 PF03099: BPL_LplA_LipB" amino acids 84 to 208 (125 residues), 101.5 bits, see alignment E=5.4e-33 PF02237: BPL_C" amino acids 273 to 317 (45 residues), 49.8 bits, see alignment 4e-17

Best Hits

Swiss-Prot: 75% identical to BIRA_SALTY: Bifunctional ligase/repressor BirA (birA) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K03524, BirA family transcriptional regulator, biotin operon repressor / biotin-[acetyl-CoA-carboxylase] ligase [EC: 6.3.4.15] (inferred from 100% identity to ddd:Dda3937_04217)

MetaCyc: 73% identical to DNA-binding transcriptional repressor/biotin-[acetyl-CoA-carboxylase] ligase BirA (Escherichia coli K-12 substr. MG1655)
Biotin--[acetyl-CoA-carboxylase] ligase. [EC: 6.3.4.15]

Predicted SEED Role

"Biotin--protein ligase (EC 6.3.4.9, EC 6.3.4.10, EC 6.3.4.11, EC 6.3.4.15) / Biotin operon repressor" in subsystem Biotin biosynthesis

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.4.15

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E0SGE1 at UniProt or InterPro

Protein Sequence (319 amino acids)

>DDA3937_RS01030 bifunctional biotin--[acetyl-CoA-carboxylase] ligase/biotin operon repressor BirA (Dickeya dadantii 3937)
MKDYTVPLKLISILSDGEFYSGEYLGELMGMSRAAINKHIQTIREWGLDVFTVTGKGYAL
SAPIQLLDADKIRSHIQSGNIAVLPVIDSTNQYLLDRLDSVSSGDACIAEYQNAGRGRRG
RTWFSPFGSNLYLSLYWRLEQGPAAAVGVSLVIGIVMAEVLHHLGADGVRVKWPNDLYLN
DKKLAGILVELNGRTGDAAHLVIGAGINLRMNSSGSDVINQGWINLQEAGIDIDRNMLAV
KLITELRTALVIYEQQGLAPFISRWNDLDNFYNRAVKLIVGNREITGIDKGIDSQGALLL
EQDGGIQSYIGGEISLRGW