Protein Info for DDA3937_RS00990 in Dickeya dadantii 3937

Annotation: tRNA (uridine(54)-C5)-methyltransferase TrmA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 367 TIGR02143: tRNA (uracil(54)-C(5))-methyltransferase" amino acids 10 to 366 (357 residues), 613.2 bits, see alignment E=6.3e-189 PF05958: tRNA_U5-meth_tr" amino acids 10 to 366 (357 residues), 630.9 bits, see alignment E=6.1e-194

Best Hits

Swiss-Prot: 95% identical to TRMA_DICC1: tRNA/tmRNA (uracil-C(5))-methyltransferase (trmA) from Dickeya chrysanthemi (strain Ech1591)

KEGG orthology group: K00557, tRNA (uracil-5-)-methyltransferase [EC: 2.1.1.35] (inferred from 100% identity to ddd:Dda3937_03896)

MetaCyc: 82% identical to tRNA m5U54 methyltransferase (Escherichia coli K-12 substr. MG1655)
tRNA (uracil-5-)-methyltransferase. [EC: 2.1.1.35]

Predicted SEED Role

"tRNA (Uracil54-C5-)-methyltransferase (EC 2.1.1.35)" (EC 2.1.1.35)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.35

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E0SGD7 at UniProt or InterPro

Protein Sequence (367 amino acids)

>DDA3937_RS00990 tRNA (uridine(54)-C5)-methyltransferase TrmA (Dickeya dadantii 3937)
MTPEILPIDQYDAQLEEKTGRLGAMMAPFDAPAPTVFRSPVSHYRMRAEFRIWHDGDDLY
HIIFDQQTKQRIRVDRFPAASELINRLMPALISALRAEPVLRRKLFQIDYLSTLSGEIVV
SLLYHKALDDEWQQCARLLRDQLHAQGFALQLIGRATKTKICLDRDYVDECLPVAGRQMI
YRQVENSFTQPNAMMNIQMLEWALSATQGSTGDLLELYCGNGNFSLALARNFERVLATEI
AKPSVAAAQYNIAANQIDNVQIIRMAAEEFTQAMNGVRQFNRLQGIDLAGYRCETIFVDP
PRSGLDDDTVKLVQAYPRILYISCNPETLCANLETLSQTHRISQLALFDQFPYTHHMECG
VLLEKRQ