Protein Info for DDA3937_RS00890 in Dickeya dadantii 3937

Annotation: glycerol kinase GlpK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 503 TIGR01311: glycerol kinase" amino acids 6 to 498 (493 residues), 753.8 bits, see alignment E=3.2e-231 PF00370: FGGY_N" amino acids 7 to 254 (248 residues), 305.8 bits, see alignment E=2.4e-95 PF02782: FGGY_C" amino acids 264 to 452 (189 residues), 155.5 bits, see alignment E=1.6e-49

Best Hits

Swiss-Prot: 87% identical to GLPK_SALPK: Glycerol kinase (glpK) from Salmonella paratyphi A (strain AKU_12601)

KEGG orthology group: K00864, glycerol kinase [EC: 2.7.1.30] (inferred from 100% identity to ddd:Dda3937_03876)

MetaCyc: 86% identical to glycerol kinase (Escherichia coli K-12 substr. MG1655)
Glycerol kinase. [EC: 2.7.1.30]

Predicted SEED Role

"Glycerol kinase (EC 2.7.1.30)" in subsystem Glycerol and Glycerol-3-phosphate Uptake and Utilization or Glycerolipid and Glycerophospholipid Metabolism in Bacteria or MLST (EC 2.7.1.30)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.30

Use Curated BLAST to search for 2.7.1.30

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E0SFN0 at UniProt or InterPro

Protein Sequence (503 amino acids)

>DDA3937_RS00890 glycerol kinase GlpK (Dickeya dadantii 3937)
MNQEKKYIVALDQGTTSSRAVVLDHDANIVSVSQREFTQIYPKAGWVEHDPMEIWASQSS
TLVEALAKAGISSDEVAGIGITNQRETAVVWEKDTGKPIYNAIVWQCRRSADICEKLKKD
GLEEYIRANTGLVVDPYFSGTKVKWILDHVEGSRERARRGELLFGTIDTWLIWKMTQGRV
HVTDYTNASRTMLFNIHKLDWDERMLEVLDIPRAMLPQVRPSSEIYGQTNIGGKGGTRIP
IAGIAGDQQAALYGQLCVHPGMAKNTYGTGCFLLMNTGTEAVRSRHGLLTTIACGPRGEV
NYALEGAVFIGGASIQWLRDELKLIGDAMDSEYFATKVKDSNGVYVVPAFTGLGAPYWDP
YARGAIFGLTRGVNANHIIRATLESIAYQTRDVLDAMQADADTRLQSLRVDGGAVANNFL
MQFQSDILGTRVERPQVRESTALGAAFLAGLATGFWNDLDEVKSKTAIEREFRPSIETVE
RNFRYRGWQKAVERARAWEEHDE