Protein Info for DDA3937_RS00180 in Dickeya dadantii 3937

Annotation: bifunctional GTP diphosphokinase/guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 TIGR00691: RelA/SpoT family protein" amino acids 26 to 697 (672 residues), 952.5 bits, see alignment E=6.8e-291 PF13328: HD_4" amino acids 26 to 175 (150 residues), 180.8 bits, see alignment E=4.8e-57 PF01966: HD" amino acids 45 to 144 (100 residues), 52 bits, see alignment E=2.5e-17 PF04607: RelA_SpoT" amino acids 235 to 345 (111 residues), 146.8 bits, see alignment E=8.8e-47 PF02824: TGS" amino acids 388 to 447 (60 residues), 73.9 bits, see alignment 2.5e-24 PF19296: RelA_AH_RIS" amino acids 459 to 548 (90 residues), 37.8 bits, see alignment E=6.7e-13 PF13291: ACT_4" amino acids 620 to 697 (78 residues), 61.3 bits, see alignment E=3.4e-20

Best Hits

Swiss-Prot: 74% identical to SPOT_VIBCH: Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (spoT) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K01139, guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase [EC: 3.1.7.2] (inferred from 100% identity to ddd:Dda3937_01127)

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2)" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5, EC 3.1.7.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.6.5

Use Curated BLAST to search for 2.7.6.5 or 3.1.7.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E0SEK2 at UniProt or InterPro

Protein Sequence (700 amino acids)

>DDA3937_RS00180 bifunctional GTP diphosphokinase/guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase (Dickeya dadantii 3937)
MYLFESLNLLIQRYLPEDQIKRLKQAYLVARDAHEGQTRSSGEPYITHPVAVACILAEMR
LDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSKLDKLKFRDKKEAQAENFR
KMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIARETLEIYSPLAHRLGIHHLKT
ELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILSEIEGRLKEAGISCRVSGREKHL
YSIYCKMHLKEQRFHSIMDIYAFRVIVREVDTCYRVLGQVHSLYKPRPGRVKDYIAIPKA
NGYQSLHTSLIGPHGVPVEVQIRTEDMDQMAEMGVAAHWAYKEGESSSTTAQVRAQRWMQ
SLLELQQSAGSSFEFIESVKSDLFPDEIYVFTPEGRIVELPAGATPVDFAYAVHTDIGHA
CVGARVDRQPYPLSQALSSGQTIEIITAPGARPNAAWLNFVVSSKARAKIRQMLKNLKRD
DSVSLGRRLINHALGSGRKLSDIPEQNIQRELERMKLASLDDLLAEIGLGNAMSVVVARN
LLEEHAETGAAGIRKLPIKGADGVLITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNI
RGYQKEPEKFMAVEWDEVTEQEFMTEIKVDMFNHQGALANLTAAISSSNSNIQSINTEER
DGRVYSAFIRLTTRDRIHLANIMRKIRVMPDVIKVNRNRN