Protein Info for COO64_RS22720 in Pseudomonas sp. SVBP6

Name: bioF
Annotation: 8-amino-7-oxononanoate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 90% identical to BIOF_PSEPW: 8-amino-7-oxononanoate synthase (bioF) from Pseudomonas putida (strain W619)

KEGG orthology group: K00652, 8-amino-7-oxononanoate synthase [EC: 2.3.1.47] (inferred from 90% identity to ppw:PputW619_4839)

MetaCyc: 38% identical to 2-amino-3-ketobutyrate CoA ligase (Escherichia coli K-12 substr. MG1655)
Glycine C-acetyltransferase. [EC: 2.3.1.29]

Predicted SEED Role

"8-amino-7-oxononanoate synthase (EC 2.3.1.47)" in subsystem Biotin biosynthesis (EC 2.3.1.47)

MetaCyc Pathways

biotin biosynthesis I (13/15 steps found)
8-amino-7-oxononanoate biosynthesis I (9/11 steps found)
8-amino-7-oxononanoate biosynthesis IV (4/5 steps found)
superpathway of L-threonine metabolism (13/18 steps found)
biotin biosynthesis II (4/6 steps found)
8-amino-7-oxononanoate biosynthesis II (1/2 steps found)
8-amino-7-oxononanoate biosynthesis III (1/2 steps found)
L-threonine degradation II (1/2 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.29 or 2.3.1.47

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (390 amino acids)

>COO64_RS22720 8-amino-7-oxononanoate synthase (Pseudomonas sp. SVBP6)
MAFDLRARLEQRRAADLYRRRPLLESPQGPEVVVDGQPLLAFCSNDYLGLANHPQVIAAW
RAGAERWGVGGGASHLVIGHSSPHHAVEEALAELTGRPRALLFSTGYMANLGALTALVGQ
GDTVLQDRLNHASLLDAGLLSGARFNRYLHNDAESLANRLDKAVGNTLVVTDGVFSMDGD
LADLPALAAVANARDAWLMVDDAHGLGTLGRNGGGIVEHFGLSLEQVPVLIGTLGKACGT
AGAFVAGSDELIECLIQFARPYIYTTSQPPALACATLRSLELLRGEHWRREHLAALIQQF
RSGAEQIGLQLMDSFTPIQPILIGDSTRALRLSQMLRERGLLVTAIRPPTVPAGSARLRV
TLSAAHSPAQVQLLLNALADCYPLLEPADA