Protein Info for COO64_RS18800 in Pseudomonas sp. SVBP6

Annotation: efflux RND transporter periplasmic adaptor subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 392 signal peptide" amino acids 1 to 31 (31 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 37 to 383 (347 residues), 165.9 bits, see alignment E=5.6e-53 PF16576: HlyD_D23" amino acids 47 to 265 (219 residues), 66.2 bits, see alignment E=5.5e-22 PF13533: Biotin_lipoyl_2" amino acids 64 to 103 (40 residues), 27.2 bits, see alignment 5.2e-10 PF13437: HlyD_3" amino acids 186 to 266 (81 residues), 39.4 bits, see alignment E=1.8e-13

Best Hits

KEGG orthology group: K13888, macrolide-specific efflux protein MacA (inferred from 82% identity to ppg:PputGB1_3781)

Predicted SEED Role

"pyoverdine-specific efflux macA-like protein" in subsystem Siderophore Pyoverdine

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (392 amino acids)

>COO64_RS18800 efflux RND transporter periplasmic adaptor subunit (Pseudomonas sp. SVBP6)
MRRSSTRRRAALYSLGLLSLGALLAWTTLPAGSDPLSTVAVTRGDIESSVTALGTLQPRR
YVDVGAQASGQIRKLHVEVGDQVKEGQLLVEIDPSTQQAKLDAGRFSIENLNAQLDEQKA
QYQLAEQQYKRQRNLAAGGATREEDLQSAQAQLKVTQARIDMYRAQIRQAQANLRSDEAE
LGYTRIYAPMSGTVVAVDAREGQTLNAQQQTPLILRIAKLSPMTVWAQVSEADIGHVKPG
MTAYFTTLAGGKRRWASSVRQILPVPPKPLDQTSQGGGSPASASGSGASSKVVLYTVLLD
VDNPDNALMAQMTTQVFFVAGRASKVLTVPVAALDEHADGNDLRIAQVLDSNGTVNQRQV
RTGLSDRLRVQVLDGLTEGERLLIGAPAASGG