Protein Info for COO64_RS10040 in Pseudomonas sp. SVBP6

Name: pncB
Annotation: nicotinate phosphoribosyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01514: nicotinate phosphoribosyltransferase
  • amino acids 10 to 399 (390 residues), 517.6 bits, E=1.2e-159
• PF17767: NAPRTase_N
  • amino acids 15 to 133 (119 residues), 125.1 bits, E=2.1e-40
• PF04095: NAPRTase
  • amino acids 173 to 399 (227 residues), 304.9 bits, E=4.8e-95

Best Hits

Swiss-Prot: 85% identical to PNCB_PSEMY: Nicotinate phosphoribosyltransferase (pncB) from Pseudomonas mendocina (strain ymp)

KEGG orthology group: K00763, nicotinate phosphoribosyltransferase [EC: 2.4.2.11] (inferred from 89% identity to ppw:PputW619_2245)

MetaCyc: 53% identical to nicotinate phosphoribosyltransferase (Escherichia coli K-12 substr. MG1655)
NICOTINATEPRIBOSYLTRANS-RXN [EC: 6.3.4.21]

Predicted SEED Role

"Nicotinate phosphoribosyltransferase (EC 2.4.2.11)" in subsystem NAD and NADP cofactor biosynthesis global or NAD regulation or Redox-dependent regulation of nucleus processes (EC 2.4.2.11)

MetaCyc Pathways

• NAD salvage pathway I (PNC VI cycle) (5/7 steps found)
• NAD salvage pathway V (PNC V cycle) (3/5 steps found)
• NAD salvage (plants) (5/11 steps found)
• superpathway of NAD biosynthesis in eukaryotes (7/14 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Nicotinate and nicotinamide metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.4.2.11, 6.3.4.21

Use Curated BLAST to search for 2.4.2.11 or 6.3.4.21

Protein Sequence (401 amino acids)

>COO64_RS10040 nicotinate phosphoribosyltransferase (Pseudomonas sp. SVBP6)
MSESVFGPSIIQNLLDTDFYKITMMQAVLHNYPNAEVEWEFRCRNSEDLTPYLAEIRYQI
EQLAEVSITQDQLAYLGRIPFIKPDFIRFLSLFRFNLRYVHVGVDDAGQLAIRLRGPWLH
VILYEIPLLAIVSEVRNRYRYREVVMEQVGERLYEKLDWLKAEASPAELAGLQLADFGTR
RRFSYRVQEHVVHTLKQDFPGRFVGTSNVHLARELDIKPIGTMAHEWFMAHQQLGPRLID
SQVAALECWVKEYRGLLGIALTDCIGMDAFLKDCDLYFAKLFDGLRHDSGDPLLWANKAI
AHYEKLGIDPKSKTLVFSDGLNFAKTLHLYRELSPRINVSFGIGTNLTCDIPGVEPMNIV
IKMTACNGAPVAKISDSPGKTQCRDENFVSYLKHVFKVEGQ