Protein Info for COO64_RS01350 in Pseudomonas sp. SVBP6

Annotation: NorM family multidrug efflux MATE transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 463 transmembrane" amino acids 12 to 32 (21 residues), see Phobius details amino acids 52 to 75 (24 residues), see Phobius details amino acids 88 to 109 (22 residues), see Phobius details amino acids 128 to 147 (20 residues), see Phobius details amino acids 159 to 179 (21 residues), see Phobius details amino acids 190 to 212 (23 residues), see Phobius details amino acids 242 to 266 (25 residues), see Phobius details amino acids 273 to 296 (24 residues), see Phobius details amino acids 317 to 336 (20 residues), see Phobius details amino acids 351 to 372 (22 residues), see Phobius details amino acids 392 to 415 (24 residues), see Phobius details amino acids 421 to 441 (21 residues), see Phobius details PF01554: MatE" amino acids 18 to 176 (159 residues), 88 bits, see alignment E=3e-29 amino acids 244 to 410 (167 residues), 101.6 bits, see alignment E=1.8e-33 TIGR00797: MATE efflux family protein" amino acids 18 to 423 (406 residues), 237.8 bits, see alignment E=1e-74

Best Hits

Swiss-Prot: 82% identical to NORM_PSEPK: Probable multidrug resistance protein NorM (norM) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K03327, multidrug resistance protein, MATE family (inferred from 83% identity to ppw:PputW619_0208)

Predicted SEED Role

"Multidrug and toxin extrusion (MATE) family efflux pump YdhE/NorM, homolog"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (463 amino acids)

>COO64_RS01350 NorM family multidrug efflux MATE transporter (Pseudomonas sp. SVBP6)
MQAAPTHELKALLRLAGPLIASQLAHMLMVLTDTLMMARISPQALAGGGLGAASYSFVSI
FCLGVIAAVGTLVAIRKGAGDIVGATRLTQAGLWLAWVMALVTALALWNLEPVLLLLGQS
PNNVEAAAQFLLLLPFALPGYLSFMALRGFTSAIGRSTPVMVISLIGTVANFLLNYALIE
GMFGLPKLGLVGIGLVTAIVANCMAVAMALYIRWHPAYAPYPISQGLSRPSWPALRELWR
LGLPIGGTYTVEVGLFAFAALCMGVLGSTELAAHQIALQIVSTAFMIPAGLSYAVTMRVG
LYYGADNLLGARHAGRVGIGFGAAMMLMFAILFWLLPDPLVGLFLDHDDPAFAAIIQLAV
SLLMVAAWFELFDGVQTIAMGSIRGLKDAKTTFLVGLFCYWVIGAPAAWLLAFTLGLGAV
GVWWGLALGLACAAVSLTLAFEWRVKRLLGSSGGVNKATASVV