Protein Info for CCNA_02600 in Caulobacter crescentus NA1000 Δfur

Annotation: S-formylglutathione hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02821: S-formylglutathione hydrolase
  • amino acids 4 to 277 (274 residues), 393.6 bits, E=2.8e-122
• PF07224: Chlorophyllase
  • amino acids 22 to 160 (139 residues), 20.8 bits, E=1.9e-08
• PF00756: Esterase
  • amino acids 23 to 271 (249 residues), 186.9 bits, E=5.4e-59

Best Hits

Swiss-Prot: 53% identical to SFGH_PARDP: S-formylglutathione hydrolase (fghA) from Paracoccus denitrificans (strain Pd 1222)

KEGG orthology group: K01070, S-formylglutathione hydrolase [EC: 3.1.2.12] (inferred from 100% identity to ccr:CC_2515)

MetaCyc: 51% identical to S-formylglutathione hydrolase / S-lactoylglutathione hydrolase (Escherichia coli K-12 substr. MG1655)
Hydroxyacylglutathione hydrolase. [EC: 3.1.2.6]; S-formylglutathione hydrolase. [EC: 3.1.2.6, 3.1.2.12]

Predicted SEED Role

"S-formylglutathione hydrolase (EC 3.1.2.12)" in subsystem Glutathione-dependent pathway of formaldehyde detoxification (EC 3.1.2.12)

MetaCyc Pathways

• formaldehyde oxidation II (glutathione-dependent) (3/3 steps found)
• methylglyoxal degradation I (2/3 steps found)
• methylglyoxal degradation VIII (2/3 steps found)
• superpathway of methylglyoxal degradation (5/8 steps found)
• superpathway of C1 compounds oxidation to CO₂ (4/12 steps found)

KEGG Metabolic Maps

• Methane metabolism
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.1.2.6

Use Curated BLAST to search for 3.1.2.12 or 3.1.2.6

Sequence Analysis Tools

See A0A0H3CB24 at UniProt or InterPro

Protein Sequence (277 amino acids)

>CCNA_02600 S-formylglutathione hydrolase (Caulobacter crescentus NA1000 Δfur)
MTVETLSTHRVHGGTLRYCRHDSTSTGTPMKFTVWIPDGQGPFPYLVWLSGLTCTEDNFT
VKSGVYEHAARHGIAIVAPDTSPRGEGVADDPAYDLGQGAGFYVDATQAPWAPHFMMHSY
VTGDLLAAVEGAFPLDGARKGIFGHSMGGHGALTIALKHPELFKSVSAFSPIVSPLNCPW
GDKALTAYLGPDRAAWRPYDACALIEDGKGASFDDILIDQGLADSFLENQLKPQLIEAAA
AKAGRKVTVRRQDGYDHSYFFISTFIGDHLAFHAQRL