Protein Info for CCNA_02076 in Caulobacter crescentus NA1000 Δfur

Annotation: protein-L-isoaspartate O-methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 222 PF01135: PCMT" amino acids 18 to 216 (199 residues), 194.7 bits, see alignment E=1.8e-61 TIGR00080: protein-L-isoaspartate O-methyltransferase" amino acids 18 to 219 (202 residues), 195.6 bits, see alignment E=5e-62 PF00398: RrnaAD" amino acids 79 to 128 (50 residues), 25.8 bits, see alignment E=5.5e-10

Best Hits

Swiss-Prot: 100% identical to PIMT_CAUVC: Protein-L-isoaspartate O-methyltransferase (pcm) from Caulobacter vibrioides (strain ATCC 19089 / CB15)

KEGG orthology group: K00573, protein-L-isoaspartate(D-aspartate) O-methyltransferase [EC: 2.1.1.77] (inferred from 100% identity to ccr:CC_1997)

MetaCyc: 48% identical to protein-L-isoaspartate O-methyltransferase (Escherichia coli K-12 substr. MG1655)
Protein-L-isoaspartate(D-aspartate) O-methyltransferase. [EC: 2.1.1.77]

Predicted SEED Role

"Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77)" in subsystem Ton and Tol transport systems (EC 2.1.1.77)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.77

Use Curated BLAST to search for 2.1.1.77

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B8GX51 at UniProt or InterPro

Protein Sequence (222 amino acids)

>CCNA_02076 protein-L-isoaspartate O-methyltransferase (Caulobacter crescentus NA1000 Δfur)
MSGGTTKAAENAKADLPRLMKALRDQGVTDPQVLKAIETTPRDLFTPDLFKDRSWEDSAL
PIACGQTISQPYIVGLMTQALTVEPRSRVLEIGTGSGYQTTILSKVSRLVYTIERYRTLM
KEAEARFNTLGLTNVITKFGDGGEGWAEQAPFDRIMVTAAAEDDPKRLLSQLKPNGVLVA
PVGKGPVQSLRRYAGDGKGGFRVEILCDVRFVPLLAGVAKDQ