Protein Info for CCNA_01961 in Caulobacter crescentus NA1000 Δfur

Annotation: biotin carboxylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00289: Biotin_carb_N
  • amino acids 1 to 110 (110 residues), 155.9 bits, E=1.2e-49
• TIGR00514: acetyl-CoA carboxylase, biotin carboxylase subunit
  • amino acids 1 to 444 (444 residues), 724 bits, E=3.5e-222
• PF02786: CPSase_L_D2
  • amino acids 115 to 321 (207 residues), 276.2 bits, E=4.2e-86
• PF02222: ATP-grasp
  • amino acids 137 to 291 (155 residues), 45.5 bits, E=1.8e-15
• PF07478: Dala_Dala_lig_C
  • amino acids 140 to 290 (151 residues), 39.6 bits, E=1e-13
• PF02785: Biotin_carb_C
  • amino acids 335 to 441 (107 residues), 120.9 bits, E=6.6e-39

Best Hits

Swiss-Prot: 60% identical to ACCC_HAEIN: Biotin carboxylase (accC) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

KEGG orthology group: K01961, acetyl-CoA carboxylase, biotin carboxylase subunit [EC: 6.3.4.14 6.4.1.2] (inferred from 100% identity to ccs:CCNA_01961)

MetaCyc: 64% identical to biotin carboxylase (Escherichia coli K-12 substr. MG1655)
Biotin carboxylase. [EC: 6.3.4.14]

Predicted SEED Role

"Biotin carboxylase of acetyl-CoA carboxylase (EC 6.3.4.14)" in subsystem Fatty Acid Biosynthesis FASII (EC 6.3.4.14)

MetaCyc Pathways

• biotin-carboxyl carrier protein assembly (4/4 steps found)
• 3-hydroxypropanoate cycle (9/13 steps found)
• superpathway of the 3-hydroxypropanoate cycle (12/18 steps found)
• fatty acid biosynthesis initiation (type I) (1/3 steps found)
• glyoxylate assimilation (8/13 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (11/18 steps found)
• jadomycin biosynthesis (3/9 steps found)
• superpathway of mycolate biosynthesis (22/239 steps found)

KEGG Metabolic Maps

• Fatty acid biosynthesis
• Propanoate metabolism
• Pyruvate metabolism
• Tetracycline biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 6.3.4.14, 6.4.1.2

Use Curated BLAST to search for 6.3.4.14 or 6.4.1.2

Sequence Analysis Tools

See A0A0H3CAS3 at UniProt or InterPro

Protein Sequence (448 amino acids)

>CCNA_01961 biotin carboxylase (Caulobacter crescentus NA1000 Δfur)
MFDKILIANRGEIALRVHRACKEMGIATVAVHSEADRNSMWVRLADESVCIGPAPAAKSY
LNIPSIIAAAEITGAQAIHPGYGFLSENARFAEIVGAHGFTFIGPKPEHIRMMGDKITAK
QAVKDAGIPVVPGSDGGVSTEEEAFEAAEKIGFPVLIKAAAGGGGRGMKVAQTREDLAEA
VSTARAEARAAFGDDTVYMERYLQKPRHIELQVIADSHGNVVHLGERDCSLQRRHQKVLE
EAPSPALSAEGRAKIGKVVVDAVKAIGYLGVGTIEFLWENDEFFFIEMNTRLQVEHPVTE
AITGIDLVREQIRIAAGLPLSFTQDDVVFEGHAIECRINAENARTFTPSPGVITDFHAPG
GLGVRLDSAIYTGYAIPPYYDSLIGKLIVHGRDRAECIARLKRCLGEMVVGGVETTIPLF
QDLLVQPDILAGDYNIHWLERWIKAQEA