Protein Info for CCNA_01809 in Caulobacter crescentus NA1000 Δfur

Annotation: lipoic acid synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 325 PF16881: LIAS_N" amino acids 17 to 69 (53 residues), 34.1 bits, see alignment 3.2e-12 TIGR00510: lipoyl synthase" amino acids 26 to 314 (289 residues), 380.5 bits, see alignment E=3e-118 PF04055: Radical_SAM" amino acids 84 to 246 (163 residues), 78.1 bits, see alignment E=9.5e-26

Best Hits

Swiss-Prot: 100% identical to LIPA_CAUVN: Lipoyl synthase (lipA) from Caulobacter vibrioides (strain NA1000 / CB15N)

KEGG orthology group: K03644, lipoic acid synthetase [EC: 2.8.1.8] (inferred from 100% identity to ccs:CCNA_01809)

MetaCyc: 52% identical to lipoyl synthase (Escherichia coli K-12 substr. MG1655)
Lipoyl synthase. [EC: 2.8.1.8]

Predicted SEED Role

"Lipoate synthase" in subsystem Lipoic acid metabolism

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.1.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B8GW82 at UniProt or InterPro

Protein Sequence (325 amino acids)

>CCNA_01809 lipoic acid synthetase (Caulobacter crescentus NA1000 Δfur)
MATVIDTLKARGSEDRAARHPEKQNRPDTPVLRKPEWLRVRAPGSGQYNETKGIVREHKL
HTVCEEAACPNIGECWSQKHATMMIMGEICTRACAFCNVTTGLPTQLDPDEPRRVAEAVA
KMGLKHVVITSVDRDDLLDGGARHFAEVVTSIRAAAPGTTIEILTPDFLRKDGAENVVID
SKPDVFNHNLETVPRLYLKIRPGARYYNSLRLLDRVKQRDPSQFTKSGLMVGLGETKEEV
MQVMDDMRSAGVDFITIGQYLQPTRKHAAIDRFVTPEEFKAYEAIARAKGFLMVSSSPLT
RSSHHAGEDFAKLQAARRALDARTA