Protein Info for CCNA_01419 in Caulobacter crescentus NA1000 Δfur

Annotation: serine hydroxymethyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00464: SHMT
  • amino acids 15 to 391 (377 residues), 566.7 bits, E=3.3e-174
• PF00155: Aminotran_1_2
  • amino acids 97 to 376 (280 residues), 29.3 bits, E=7.7e-11

Best Hits

Swiss-Prot: 100% identical to GLYA_CAUVC: Serine hydroxymethyltransferase (glyA) from Caulobacter vibrioides (strain ATCC 19089 / CB15)

KEGG orthology group: K00600, glycine hydroxymethyltransferase [EC: 2.1.2.1] (inferred from 100% identity to ccr:CC_1357)

MetaCyc: 71% identical to serine hydroxymethyltransferase 1 subunit (Sinorhizobium meliloti Rm2011)
Glycine hydroxymethyltransferase. [EC: 2.1.2.1]

Predicted SEED Role

"Serine hydroxymethyltransferase (EC 2.1.2.1)" in subsystem Folate Biosynthesis or Glycine Biosynthesis or Glycine and Serine Utilization or LMPTP YwlE cluster or Photorespiration (oxidative C2 cycle) or Serine-glyoxylate cycle or Serine Biosynthesis (EC 2.1.2.1)

MetaCyc Pathways

• folate transformations III (E. coli) (8/9 steps found)
• superpathway of L-serine and glycine biosynthesis I (4/4 steps found)
• dTMP de novo biosynthesis (mitochondrial) (3/3 steps found)
• glycine degradation (3/3 steps found)
• folate polyglutamylation (4/5 steps found)
• glycine biosynthesis I (1/1 steps found)
• folate transformations II (plants) (8/11 steps found)
• glycine betaine degradation III (4/7 steps found)
• folate transformations I (8/13 steps found)
• formaldehyde assimilation I (serine pathway) (8/13 steps found)
• photorespiration I (5/9 steps found)
• photorespiration III (5/9 steps found)
• glycine betaine degradation I (4/8 steps found)
• glycine betaine degradation II (mammalian) (1/4 steps found)
• photorespiration II (5/10 steps found)
• purine nucleobases degradation II (anaerobic) (14/24 steps found)

KEGG Metabolic Maps

• Cyanoamino acid metabolism
• Glycine, serine and threonine metabolism
• Methane metabolism
• One carbon pool by folate

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.2.1

Sequence Analysis Tools

See A0A0H3C7W2 at UniProt or InterPro

Protein Sequence (428 amino acids)

>CCNA_01419 serine hydroxymethyltransferase (Caulobacter crescentus NA1000 Δfur)
MTQTDLSAFFGADLATADRDIFDRIGRELGRQQNQIELIASENIVSKAVLEAQGSILTNK
YAEGYPGKRYYGGCEYVDEIETIAIERAKALFGAGFANVQPHSGSQANQAVFMALLQPGD
TFLGMDLAAGGHLTHGSPANQSGKWFKPISYSVRQQDQLIDYDGVAEVAQREKPKLIIAG
GSAYSREIDFAKFREIADSIGAYLMVDMAHYAGLIAGGAYANPIPHAHIVTTTTHKTLRG
PRGGLVLTNDEAIIKKVNSAVFPGLQGGPLEHVIAAKAVAFGEALQPSFKDYARQVVANA
RALAEALLKSGVNIVSGGTDSHLMLVDLRPKGVTGRDAEHSLERAYMTCNKNGVPFDTAP
FTITSGIRLGTPAGTTRGFKEAEFTRVGELIGEVVNGLAVNGPEGNAAVEAKVREEVLAL
TGRFPIYN