Protein Info for CCNA_00850 in Caulobacter crescentus NA1000 Δfur

Annotation: cation/multidrug efflux pump acrB2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1051 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details transmembrane" amino acids 341 to 360 (20 residues), see Phobius details amino acids 367 to 389 (23 residues), see Phobius details amino acids 395 to 416 (22 residues), see Phobius details amino acids 439 to 460 (22 residues), see Phobius details amino acids 471 to 493 (23 residues), see Phobius details amino acids 542 to 561 (20 residues), see Phobius details amino acids 873 to 891 (19 residues), see Phobius details amino acids 898 to 918 (21 residues), see Phobius details amino acids 924 to 948 (25 residues), see Phobius details amino acids 969 to 989 (21 residues), see Phobius details amino acids 1001 to 1026 (26 residues), see Phobius details TIGR00915: RND transporter, hydrophobe/amphiphile efflux-1 (HAE1) family" amino acids 2 to 1044 (1043 residues), 1729.1 bits, see alignment E=0 PF00873: ACR_tran" amino acids 3 to 1027 (1025 residues), 1350.4 bits, see alignment E=0 PF03176: MMPL" amino acids 339 to 500 (162 residues), 27.3 bits, see alignment E=2.6e-10 amino acids 826 to 1036 (211 residues), 23.3 bits, see alignment E=4.5e-09

Best Hits

Swiss-Prot: 66% identical to ACRB_ECOLI: Multidrug efflux pump subunit AcrB (acrB) from Escherichia coli (strain K12)

KEGG orthology group: K03296, hydrophobic/amphiphilic exporter-1 (mainly G- bacteria), HAE1 family (inferred from 68% identity to abo:ABO_0964)

MetaCyc: 66% identical to multidrug efflux pump RND permease AcrB (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-354; TRANS-RXN-355; TRANS-RXN-356; TRANS-RXN-357; TRANS-RXN-359; TRANS-RXN0-592

Predicted SEED Role

"RND efflux system, inner membrane transporter CmeB" in subsystem Multidrug Resistance Efflux Pumps or Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0H3C4R5 at UniProt or InterPro

Protein Sequence (1051 amino acids)

>CCNA_00850 cation/multidrug efflux pump acrB2 (Caulobacter crescentus NA1000 Δfur)
MLSRFFIDRPIFAWVIAIVIMLAGALAIRTLPIAQYPEIALPQVSISANYPGASAKTVED
SVTQVIEQKMKGLDGLDYMSSTSDGSGSATVTLTFKAGTDIDIAQVQVQNKLQTATALLP
QEVQQQGLTVAKSARNFMMVIGLYSEDPKTTGADLADYMASNLQDPLSRVDGVGDIQLFG
SQYAMRIWLDPQKLASFSLTPADVAAAIRAQNAQVSAGQIGGTPNLPGTGLNATITAQSR
LQTPEQFRQIIVKNTSGGATVRLSDVARVELGAESYASIAKFNGYQAAGMAIKLAPGANA
LDTAAAVNARMAQLEKNFPANYKYVIPYDSTPFVKLSIEEVVKTLVEAIILVFIVMFLFL
QNWRATLIPTIAVPVVLLGTFGVLAAFGYSINTLTMFGLVLAIGLLVDDAIVVVENVERV
MSEEGLSPKEATRKSMNEITGALIGIALVLAAVFVPMAFFGGSQGVIYRQFSITIVSAMA
LSVVVALVLTPALTATMLKPVKAGHHEEKTGFFGWFNRSFNDMSSRYQGSVRKILGKRGR
WMAIYAAIIVAMGLLFVRLPSAFLPEEDQGTMFTLVQLPAGATEEKTLAVLDQVRDHFLV
GEKEAVQSVFTVSGFSFAGAGQNAGLAFVRLKDFEHRKSANLKAQAVAGRAMGAFSQFRD
AMVFAIVPPAVQELGNSSGFDFQLQDVGGVGHEALMNARNMMLGMASQDPSLVGVRPNGQ
DDTPQLKIEVDQAKAGALGLTTADINSALSAAWGGSYVNDFIDRGRVKKVYMQADAPFRM
TPEDLNRWYVRNNQGQMVAFPAFATASWTYGSPRLERYNGLSSVNIQGAPAPGKSSGDAI
AAMEKIAAKLPPGVGYEWTGLSAQELEAGNQAPALYAISILVVFLLLAALYESWSIPLAV
IMVIPLGVIGALLATFARGLSNDIYFQVGLLTTMGLAAKNAILIVEFAKDLYEKGMGLIE
ATLEAVRLRLRPIIMTSLAFVFGVLPLAISNGAGSGAQHAIGTGVIGGMISATLLAIFFV
PLFFVVVEKIFKPKHNGHDAAQGQVSSTEAH