Protein Info for CA264_21665 in Pontibacter actiniarum KMM 6156, DSM 19842

Annotation: 2-hydroxyacid dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00389: 2-Hacid_dh
  • amino acids 9 to 322 (314 residues), 73.9 bits, E=1.6e-24
• PF02826: 2-Hacid_dh_C
  • amino acids 109 to 297 (189 residues), 171.4 bits, E=2e-54

Best Hits

Swiss-Prot: 49% identical to LDHD_ECOLI: D-lactate dehydrogenase (ldhA) from Escherichia coli (strain K12)

KEGG orthology group: K03778, D-lactate dehydrogenase [EC: 1.1.1.28] (inferred from 58% identity to cao:Celal_2963)

MetaCyc: 49% identical to D-lactate dehydrogenase (Escherichia coli K-12 substr. MG1655)
D-lactate dehydrogenase. [EC: 1.1.1.28]

Predicted SEED Role

"D-lactate dehydrogenase (EC 1.1.1.28)" in subsystem Fermentations: Lactate or Fermentations: Mixed acid (EC 1.1.1.28)

MetaCyc Pathways

• superpathway of glucose and xylose degradation (16/17 steps found)
• L-alanine degradation II (to D-lactate) (3/3 steps found)
• pyruvate fermentation to (R)-lactate (1/1 steps found)
• vancomycin resistance I (1/3 steps found)
• mixed acid fermentation (10/16 steps found)
• heterolactic fermentation (11/18 steps found)
• L-alanine degradation VI (reductive Stickland reaction) (2/6 steps found)
• superpathway of L-alanine fermentation (Stickland reaction) (2/9 steps found)
• superpathway of fermentation (Chlamydomonas reinhardtii) (2/9 steps found)
• lactate fermentation to acetate, CO₂ and hydrogen (Desulfovibrionales) (1/8 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.28

Use Curated BLAST to search for 1.1.1.28

Sequence Analysis Tools

See A0A1X9YZ27 at UniProt or InterPro

Protein Sequence (332 amino acids)

>CA264_21665 2-hydroxyacid dehydrogenase (Pontibacter actiniarum KMM 6156, DSM 19842)
MRVAMYSTHTFERSFLEQANDAKHELLFLKPRLSDTTVPLAQGCQAVSIFVGDDASQPVL
EALSEIGVQFLALRSTGYNHVDLKAAAMLQMRIARVMEYSPYAVAEHSVALMLALNRKLI
KAHTRVRELNFSLDGLVGFDMHRKTVGIIGLGKIGKVVATILRGFGCGILVSDPSPDYHF
ATHLKIKYTDVDTLCTQSDIISLHAPLTAQTRYLINAERISKMKRGVMLINTSRGALVNT
KDVIQGLKTGQIGSLGLDVYEEEQNLFFEDHSEEILQDDVIARLLTFQNVLITSHQAFLT
DTALQNIARTTIYNLKCFEQGVVGVNELNEMM