Protein Info for CA264_20690 in Pontibacter actiniarum KMM 6156, DSM 19842

Annotation: methylmalonyl-CoA mutase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00641: methylmalonyl-CoA mutase N-terminal domain
  • amino acids 29 to 549 (521 residues), 906.3 bits, E=4.8e-277
• PF01642: MM_CoA_mutase
  • amino acids 30 to 543 (514 residues), 796.4 bits, E=1.1e-243
• TIGR00640: methylmalonyl-CoA mutase C-terminal domain
  • amino acids 582 to 711 (130 residues), 200.5 bits, E=9e-64
• PF02310: B12-binding
  • amino acids 585 to 697 (113 residues), 71.6 bits, E=5.2e-24

Best Hits

Swiss-Prot: 74% identical to MUTB_PORGI: Methylmalonyl-CoA mutase large subunit (mutB) from Porphyromonas gingivalis (strain ATCC BAA-308 / W83)

KEGG orthology group: K01847, methylmalonyl-CoA mutase [EC: 5.4.99.2] (inferred from 80% identity to lil:LB_274)

MetaCyc: 76% identical to methylmalonyl-CoA mutase large subunit (Chloroflexus aurantiacus)
Methylmalonyl-CoA mutase. [EC: 5.4.99.2]

Predicted SEED Role

"Methylmalonyl-CoA mutase (EC 5.4.99.2)" in subsystem Propionyl-CoA to Succinyl-CoA Module or Serine-glyoxylate cycle (EC 5.4.99.2)

MetaCyc Pathways

• propanoyl CoA degradation I (3/3 steps found)
• 2-oxobutanoate degradation I (3/4 steps found)
• pyruvate fermentation to propanoate I (4/7 steps found)
• conversion of succinate to propanoate (1/3 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (6/10 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (11/17 steps found)
• 3-hydroxypropanoate cycle (8/13 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (11/18 steps found)
• superpathway of L-methionine salvage and degradation (9/16 steps found)
• methylaspartate cycle (11/19 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (6/13 steps found)
• L-glutamate degradation VIII (to propanoate) (4/11 steps found)
• superpathway of the 3-hydroxypropanoate cycle (8/18 steps found)
• crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) (3/14 steps found)

KEGG Metabolic Maps

• Propanoate metabolism
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 5.4.99.2

Use Curated BLAST to search for 5.4.99.2

Sequence Analysis Tools

See A0A1X9YXU8 at UniProt or InterPro

Protein Sequence (714 amino acids)

>CA264_20690 methylmalonyl-CoA mutase (Pontibacter actiniarum KMM 6156, DSM 19842)
MKPDFSKIDINKVAASDKAPKDPLQTKPWKTPEQIEVKSFYTSEDAANFEHLGFAAGLPP
YLRGPYSTMYVQKPWTIRQYAGFSTAEESNAFYRRNLAGGQKGLSVAFDLATHRGYDSDH
PRVVGDVGKAGVAIDSVEDMKILFDQIPLDQMSVSMTMNGAVLPIMAFYIVAAEEQGVKP
EQLSGTIQNDILKEFMVRNTYIYPPEPSMKIIADIFEFTSKNMPRFNSISISGYHMQEAG
ATADIELAYTLADGLEYVRTGLKAGMDIDEFAPRLSFFWAIGMNHFMEIAKMRAARMLWA
KLISQFNPKNPKSLALRTHCQTSGWSLTEQDPFNNVTRTCVEAMAAALGGTQSLHTNALD
EAIALPTDFSARIARNTQIYLQQETNITKVVDPWGGSYYVEALTHEITQKAWALMQEVEE
LGGMAKAIETGLPKMRIEEAAARKQARIDSGKDIIVGVNKYKPSQIQEIDILDIDNTVVR
ESQLRRLASIKSTRDEGSVLNALQALTDAAESGVGNLLELAVEAARRRATLGEISDALEK
VYGRHKAVIRAISGVYSAELTDDDNFERAKELADQFEALEGRRPRIMVAKMGQDGHDRGS
KVIATSFADLGFDVDIGPLFQTPAEVAMQAAENDVHVVGVSSLAAGHKTLVPQLIKELRK
LGREDILVIVGGVIPAQDYDFLFKAGAIGVFGPGTIISVAAQNILEQLMKQVKS