Protein Info for CA264_20625 in Pontibacter actiniarum KMM 6156, DSM 19842

Annotation: methylcrotonoyl-CoA carboxylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 542 transmembrane" amino acids 164 to 187 (24 residues), see Phobius details PF01039: Carboxyl_trans" amino acids 37 to 534 (498 residues), 507.8 bits, see alignment E=3.1e-156

Best Hits

KEGG orthology group: None (inferred from 77% identity to phe:Phep_1612)

Predicted SEED Role

"Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism or Serine-glyoxylate cycle (EC 6.4.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.4.1.4

Use Curated BLAST to search for 6.4.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9YXG1 at UniProt or InterPro

Protein Sequence (542 amino acids)

>CA264_20625 methylcrotonoyl-CoA carboxylase (Pontibacter actiniarum KMM 6156, DSM 19842)
MDIEFNKNEDALKQLVFQLKNKLKKVHLGGGEKRIEKEHKKGKMTARERIDYLLDEGTEF
LEIGAFAGEGMYEEVGGCPSGGVVTGIGYIKGRQCVVVANDATVKAGAWFPITAKKNLRA
QEISIENKLPIVYLVDSAGVFLPMQDEIFPDKEHFGRMFRNNAVMSSMGIVQIAAIMGSC
VAGGAYLPIMSDEALIVEGTGSVFLAGSYLVKSAIGENIDNETLGGATTHSEISGVTDYK
CKDDKEALDHIRNIFDKMGDKPKAGFSRVAPAAPAEDQKEIYGLLPADRVKPYDMMDIIR
RLVDNSEFEPYKELYGQTLICGLARIDGWAVGIVANQRKIMKSKKGEMQMGGVIYSDSAD
KAARFIMNCNQKKIPLVFLQDVSGFMVGSKSEHGGIIKDGAKMVSAMSNSVVPKFTILVG
NSYGAGNYAMCGKAYDPRLIYAWPTAQLAVMSGAAAANTLLQIQVSALKAKGEEITPEAE
KELLERITNKYNEELSPYYAAARLWVDGIIDPLETRKVISMGIEAANHAPIEKPYNVGVI
QT