Protein Info for CA264_20435 in Pontibacter actiniarum KMM 6156, DSM 19842
Annotation: phosphomethylpyrimidine synthase ThiC
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 79% identical to THIC_PSEAB: Phosphomethylpyrimidine synthase (thiC) from Pseudomonas aeruginosa (strain UCBPP-PA14)
KEGG orthology group: K03147, thiamine biosynthesis protein ThiC (inferred from 82% identity to psn:Pedsa_1385)MetaCyc: 75% identical to phosphomethylpyrimidine synthase (Escherichia coli K-12 substr. MG1655)
PYRIMSYN1-RXN [EC: 4.1.99.17]
Predicted SEED Role
"Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17)" (EC 4.1.99.17)
MetaCyc Pathways
- superpathway of thiamine diphosphate biosynthesis I (9/10 steps found)
- superpathway of thiamine diphosphate biosynthesis II (9/11 steps found)
- 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis I (2/2 steps found)
- superpathway of thiamine diphosphate biosynthesis III (eukaryotes) (3/7 steps found)
Isozymes
No predicted isozymesUse Curated BLAST to search for 4.1.99.17
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A1X9YXR4 at UniProt or InterPro
Protein Sequence (620 amino acids)
>CA264_20435 phosphomethylpyrimidine synthase ThiC (Pontibacter actiniarum KMM 6156, DSM 19842) MRQDKTPTASAITRTPFPASRKVYVPGQLHNIKVAMREITLEDTVTKFLGVEEREQNPPV TVYDTSGPFTDPEVEIDVRQGLARLREEWIVSRGDVEQLPGITSAYGQERLNDASLDALR FQHLSKPYRAKAGQNVTQLHYARKGIITPEMEYIAIRENQRLAENAEMGHQHAGNSFGAN TPKGFITPEFVRQEVAAGRAVIPSNINHPESEPMIIGRNFLVKINANIGNSAVTSSIEEE VEKAVWACRWGADTIMDLSTGKNIHETREWIIRNSPVPIGTVPIYQALEKVNGKAEDLTW EIFRDTLIEQAEQGVDYFTIHAGVLLRYVPLTANRVTGIVSRGGSIMAKWCLAHHKENFL YTHFEEICEIMKAYDVAFSLGDGLRPGSIADANDAAQFSELETLGELTKIAWKHDVQVII EGPGHIPMHLIKENMDKQLEVCGEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWYGTAM LCYVTPKEHLGLPNKKDVKDGVITYKLAAHAADLAKGHPGAQYRDNALSKARFEFRWQDQ FNLALDPDTAREYHDETLPAEGAKVAHFCSMCGPHFCSMKITQEVRDYARTKGASEEEAL QQGMAEQAETFKAKGAEIYS