Protein Info for CA264_20335 in Pontibacter actiniarum KMM 6156, DSM 19842

Annotation: isoaspartyl peptidase/L-asparaginase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 343 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF01112: Asparaginase_2" amino acids 30 to 336 (307 residues), 410.1 bits, see alignment E=2.8e-127

Best Hits

Swiss-Prot: 53% identical to IAAA_ECOLI: Isoaspartyl peptidase (iaaA) from Escherichia coli (strain K12)

KEGG orthology group: K13051, beta-aspartyl-peptidase (threonine type) [EC: 3.4.19.5] (inferred from 66% identity to lby:Lbys_1513)

MetaCyc: 53% identical to isoaspartyl dipeptidase proenzyme (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Isoaspartyl aminopeptidase (EC 3.4.19.5) @ Asp-X dipeptidase" (EC 3.4.19.5)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 3.4.19.5

Use Curated BLAST to search for 3.4.19.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9YXE3 at UniProt or InterPro

Protein Sequence (343 amino acids)

>CA264_20335 isoaspartyl peptidase/L-asparaginase (Pontibacter actiniarum KMM 6156, DSM 19842)
MKKSLYLLCLFLLTAFVSMAQGKPDYSKITLVIHGGAGTITRANMTPEKEKAYREKLNEA
LQVGYGVLKKGGTSMDAVEATIHVMENSPLFNAGKGAVFTNEGKNEMDAAMMDGKTLQAG
SVASVTTIKNPISAARAVMEKSEHVMMIGDGAEKFAKMNGVEIVDPSYFYTETRFNQLQK
IKGEEKTQLDHDGGTSSSENIFTEGNKFGTVGCVALDAYGNLAAGTSTGGMTNKRYGRVG
DAPIIGAGTYADNSTCAVSATGHGEYFIRNVVAHDIAALMAYKGWPVQKAAEEVVMKKLV
GRGGEGGVIALDKNGNVAMPFNSAGMYRGFMKNGKAEVYIYKD