Protein Info for CA264_18490 in Pontibacter actiniarum KMM 6156, DSM 19842

Annotation: dihydrolipoamide succinyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 524 PF00364: Biotin_lipoyl" amino acids 4 to 75 (72 residues), 70.7 bits, see alignment E=1.9e-23 amino acids 113 to 184 (72 residues), 67.8 bits, see alignment E=1.5e-22 TIGR01347: dihydrolipoyllysine-residue succinyltransferase, E2 component of oxoglutarate dehydrogenase (succinyl-transferring) complex" amino acids 112 to 523 (412 residues), 552 bits, see alignment E=5e-170 PF02817: E3_binding" amino acids 229 to 260 (32 residues), 43.3 bits, see alignment (E = 8.7e-15) PF00198: 2-oxoacid_dh" amino acids 294 to 522 (229 residues), 279.9 bits, see alignment E=3.8e-87

Best Hits

KEGG orthology group: K00658, 2-oxoglutarate dehydrogenase E2 component (dihydrolipoamide succinyltransferase) [EC: 2.3.1.61] (inferred from 67% identity to psn:Pedsa_2379)

Predicted SEED Role

"Dihydrolipoamide succinyltransferase component (E2) of 2-oxoglutarate dehydrogenase complex (EC 2.3.1.61)" in subsystem TCA Cycle (EC 2.3.1.61)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.61

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9YWP3 at UniProt or InterPro

Protein Sequence (524 amino acids)

>CA264_18490 dihydrolipoamide succinyltransferase (Pontibacter actiniarum KMM 6156, DSM 19842)
MSLEVKVPAVGESITEVTIAQWLKKDGDRVEMDEVIAELESDKATFELPAEAAGILRIVA
QEGDTVDVGAIICKIEENGAAAAAPSAPAAEASQPAAQEAAPAATGGAGETVEMKVPTVG
ESITEVTIASWLKSSGDHVEMDEVIAELESDKATFELPAEAAGTLEIVAQEGDTVEIGGL
LCRIVSGAGAVKANAQATAKQAAADQQAATARPAAASSGAQTYASGTPSPAAGKILSEKG
INPADVQGTGRDGRITKEDAQNAQKSAAKPAAAPAPKQEATASNDAPAAAGDRNQRREKM
SSLRKTVARRLVQVKNETAMLTTFNEVDMKPIMDIRAKYKDKFKEVHEVGLGFMSFFTKA
CTVALKEWPAVNAQIDGNDMVFNDFVDVSIAVSSPKGLVVPVIRNAEQLTLDGIEKEVIR
LAKKARDGKLSIEEMTGGTFTITNGGVFGSMMSTPIINAPQSAILGMHNIVQRPVAVNGQ
VEIRPMMYLALSYDHRIIDGRESVSFLVRVKELLEDPMRLLLGV