Protein Info for CA264_17990 in Pontibacter actiniarum KMM 6156, DSM 19842

Annotation: nitric oxide dioxygenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 403 PF00042: Globin" amino acids 26 to 130 (105 residues), 54.1 bits, see alignment E=3.3e-18 PF00970: FAD_binding_6" amino acids 158 to 259 (102 residues), 23.1 bits, see alignment E=1.1e-08 PF00175: NAD_binding_1" amino acids 271 to 374 (104 residues), 59.4 bits, see alignment E=8e-20

Best Hits

Swiss-Prot: 55% identical to HMP_BORPE: Flavohemoprotein (hmp) from Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251)

KEGG orthology group: K05916, nitric oxide dioxygenase [EC: 1.14.12.17] (inferred from 63% identity to dfe:Dfer_2185)

MetaCyc: 44% identical to nitric oxide dioxygenase (Escherichia coli K-12 substr. MG1655)
6,7-dihydropteridine reductase. [EC: 1.5.1.34]; Nitric oxide dioxygenase. [EC: 1.5.1.34, 1.14.12.17]

Predicted SEED Role

"Flavohemoprotein (Hemoglobin-like protein) (Flavohemoglobin) (Nitric oxide dioxygenase) (EC 1.14.12.17)" in subsystem Bacterial hemoglobins or Flavohaemoglobin or Glutaredoxins (EC 1.14.12.17)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.14.12.17, 1.5.1.34

Use Curated BLAST to search for 1.14.12.17 or 1.5.1.34

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9YYH4 at UniProt or InterPro

Protein Sequence (403 amino acids)

>CA264_17990 nitric oxide dioxygenase (Pontibacter actiniarum KMM 6156, DSM 19842)
MTTEQKELIKATVPILREHGVALTTHFYSRMFTHNPELKNLFNMGNQGNGKQQLSLAMAV
LAYAENIEDPSVLVSAVTKIGHKHMSLDIRPEHYAIVGKHLLASIGEVLGEGATPTLIEA
WAVAYQQLADLMIGLEQNLYSDAVAKEGGWTGWRPFLVKQKVQESEEITSFYLYPADGGK
VADFTPGQYISLRLFLPELNLLQPRQYSLSNAPNGDYYRISVKKEAGKAEHPNGMISNRL
HNAVQEGDVVEVAPPAGDFVLDTTKQTPVVFVSGGVGQTPLMSMLEYLASTQSSRDIVWV
HGSRNRKVHAFREPLTMLEAKIGKLNKHIFYDTLDEEMQVSGYYEGVVELDKLKEQALLQ
NADYYICGPAPFIQKQFTDLAAMGVSPEAIHYEEFGPANLVLS