Protein Info for CA264_17905 in Pontibacter actiniarum KMM 6156, DSM 19842

Annotation: UDP-glucose 4-epimerase GalE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01179: UDP-glucose 4-epimerase GalE
  • amino acids 5 to 341 (337 residues), 411.2 bits, E=1.2e-127
• PF04321: RmlD_sub_bind
  • amino acids 5 to 166 (162 residues), 38.1 bits, E=2.9e-13
• PF08659: KR
  • amino acids 6 to 88 (83 residues), 32.1 bits, E=3.3e-11
• PF01370: Epimerase
  • amino acids 6 to 260 (255 residues), 168.7 bits, E=4.5e-53
• PF02719: Polysacc_synt_2
  • amino acids 6 to 189 (184 residues), 43.5 bits, E=7e-15
• PF16363: GDP_Man_Dehyd
  • amino acids 7 to 330 (324 residues), 165.3 bits, E=8e-52
• PF01073: 3Beta_HSD
  • amino acids 7 to 259 (253 residues), 54.6 bits, E=2.6e-18

Best Hits

Swiss-Prot: 52% identical to GALE_BACSU: UDP-glucose 4-epimerase (galE) from Bacillus subtilis (strain 168)

KEGG orthology group: K01784, UDP-glucose 4-epimerase [EC: 5.1.3.2] (inferred from 62% identity to cao:Celal_2022)

MetaCyc: 52% identical to UDP-glucose 4-epimerase (Bacillus subtilis subtilis 168)
UDP-N-acetylglucosamine 4-epimerase. [EC: 5.1.3.7]; UDP-glucose 4-epimerase. [EC: 5.1.3.7, 5.1.3.2]

Predicted SEED Role

"UDP-glucose 4-epimerase (EC 5.1.3.2)" in subsystem Lacto-N-Biose I and Galacto-N-Biose Metabolic Pathway or Lactose and Galactose Uptake and Utilization or N-linked Glycosylation in Bacteria or Rhamnose containing glycans or linker unit-arabinogalactan synthesis (EC 5.1.3.2)

MetaCyc Pathways

• colanic acid building blocks biosynthesis (9/11 steps found)
• UDP-α-D-galactose biosynthesis (1/1 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis I (1/1 steps found)
• superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis (4/6 steps found)
• UDP-α-D-galactofuranose biosynthesis (1/2 steps found)
• D-galactose degradation I (Leloir pathway) (3/5 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (4/7 steps found)
• D-galactose detoxification (1/3 steps found)
• UDP-sugars interconversion (4/9 steps found)
• stachyose degradation (1/7 steps found)
• teichuronic acid biosynthesis (B. subtilis 168) (2/9 steps found)
• mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (1/18 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (10/33 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Galactose metabolism
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 5.1.3.2

Use Curated BLAST to search for 5.1.3.2 or 5.1.3.7

Sequence Analysis Tools

See A0A1X9YWI3 at UniProt or InterPro

Protein Sequence (344 amino acids)

>CA264_17905 UDP-glucose 4-epimerase GalE (Pontibacter actiniarum KMM 6156, DSM 19842)
MTKEKILVTGGAGYIGSHTVVELVQAGYEPVIVDNFSNSEESALEGIAAILGRSVPCHRI
DCTDTAALRRVFEQEQNIQGVIHFAAYKAVGESVAEPLKYYHNNVGSLVALLQVMEEVGV
TNLVFSSSCTVYGIPDKLPVTEETPVQKANSPYGNTKKMCEEVLTDLANSGKSSIKATAL
RYFNPIGAHPSAKIGELPLGVPNNLVPFITQTAVGMREQLTVFGDDYDTSDGTCIRDYIH
VVDLAKAHVVAVERLLQGKARAIEFFNVGTGTGNTVLEAIKAFEKASGQKLNYKVGPRRV
GDVPKIYADVSVATEELGFKTTSTLEEAMKSAWDWQLSLQKEKK