Protein Info for CA264_17895 in Pontibacter actiniarum KMM 6156, DSM 19842

Annotation: protein-(glutamine-N5) methyltransferase, release factor-specific

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 284 TIGR00536: methyltransferase, HemK family" amino acids 3 to 281 (279 residues), 210.2 bits, see alignment E=3.3e-66 PF17827: PrmC_N" amino acids 6 to 77 (72 residues), 54.4 bits, see alignment E=6.1e-18 TIGR03534: protein-(glutamine-N5) methyltransferase, release factor-specific" amino acids 28 to 280 (253 residues), 268.1 bits, see alignment E=7.7e-84 PF05175: MTS" amino acids 114 to 198 (85 residues), 45 bits, see alignment E=3.9e-15 PF13847: Methyltransf_31" amino acids 114 to 205 (92 residues), 43.8 bits, see alignment E=9.6e-15 PF03602: Cons_hypoth95" amino acids 115 to 201 (87 residues), 24.1 bits, see alignment E=1e-08 PF13649: Methyltransf_25" amino acids 118 to 203 (86 residues), 43.6 bits, see alignment E=1.6e-14 PF08241: Methyltransf_11" amino acids 119 to 190 (72 residues), 29.3 bits, see alignment E=4.5e-10 PF01170: UPF0020" amino acids 134 to 201 (68 residues), 23.9 bits, see alignment E=1.2e-08

Best Hits

Swiss-Prot: 46% identical to PRMC_BACTN: Release factor glutamine methyltransferase (prmC) from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

KEGG orthology group: K02493, methyltransferase [EC: 2.1.1.-] (inferred from 42% identity to bsa:Bacsa_2083)

Predicted SEED Role

"Protein-N(5)-glutamine methyltransferase PrmC, methylates polypeptide chain release factors RF1 and RF2"

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9YWA9 at UniProt or InterPro

Protein Sequence (284 amino acids)

>CA264_17895 protein-(glutamine-N5) methyltransferase, release factor-specific (Pontibacter actiniarum KMM 6156, DSM 19842)
MATIQQLQQHIRDKIKAAYPEPEAGSIAQLVLEHVLQKNRVQLTLAQQEEVRPEQEAQVE
LVVQRLAQQEPVQYVLGVAHFYGLELLVDQRVLIPRPETEELVDLVLREHRGQAGLRVLD
ICAGSGCIPLALAANQPNADVYGLEVSEGALQVARSNAAKYGLPVEWLQADIFEPVQRVA
AGSLDIITSNPPYVLEEEKEQMRKNVLQYEPHLALFVPNQDPLRYYRRITEVGLQLLKKG
GILYFEINERYGKAVQEFMLEAGFAEAQVVQDLFGKDRIVRAKR