Protein Info for CA264_17580 in Pontibacter actiniarum KMM 6156, DSM 19842

Annotation: glutamate--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 67% identical to SYE_CYTH3: Glutamate--tRNA ligase (gltX) from Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469)

KEGG orthology group: K01885, glutamyl-tRNA synthetase [EC: 6.1.1.17] (inferred from 67% identity to chu:CHU_3373)

Predicted SEED Role

"Glutamyl-tRNA synthetase (EC 6.1.1.17) @ Glutamyl-tRNA(Gln) synthetase (EC 6.1.1.24)" (EC 6.1.1.17, EC 6.1.1.24)

MetaCyc Pathways

tRNA charging (20/21 steps found)
superpathway of heme b biosynthesis from glutamate (10/10 steps found)
tetrapyrrole biosynthesis I (from glutamate) (6/6 steps found)
glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
(2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
adenosylcobalamin biosynthesis II (aerobic) (13/33 steps found)
adenosylcobalamin biosynthesis I (anaerobic) (13/36 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.17 or 6.1.1.24

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9YW02 at UniProt or InterPro

Protein Sequence (513 amino acids)

>CA264_17580 glutamate--tRNA ligase (Pontibacter actiniarum KMM 6156, DSM 19842)
MEREVRVRFAPSPTGPLHIGGVRTALYNFLLAKKTGGKMILRIEDTDQNRFVPGAEDYIR
ESLEWCGIELDESPWSGGPYAPYRQSERKPMYMQYALQLVNDGHAYYAFDTAEELEEMRE
RLKAAKVATPQYNAITRATMKNSLTLPEDEVKKRLESGDPYVIRLKVPRKEEVRLKDMIR
GWVMVHSSSIDDKVLMKSDGMPTYHLANIVDDHLMKITHVIRGEEWLPSAPLHVLLYRYL
GWEDTMPEFAHLPLLLKPDGNGKLSKRDGDKLGFPVFPLRWQDPVTGEISSGYRESGYLP
EAFINFLAFLGWNPGTSQEIFSMEELANEFSVERIGKSGTRFDIQKARWFNEQYLRAKPD
NELAGYLIKALAEHNITTTPEKAEKIAGLMKERVSFPQDFWQEAKYFFVAPEEYSEKVAS
KKWNSQSVAVFEDFKNELPSLENFNADTVKELLMSVLERHGMKIGQVMQALRLAVTGAEA
GPDLMHIIEVIGREETIKRIDTATSKLSSYLSA