Protein Info for CA264_17510 in Pontibacter actiniarum KMM 6156, DSM 19842

Annotation: pseudouridylate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 542 PF01479: S4" amino acids 310 to 356 (47 residues), 44.2 bits, see alignment 1.3e-15 PF00849: PseudoU_synth_2" amino acids 370 to 501 (132 residues), 84.5 bits, see alignment E=9.4e-28 TIGR00093: pseudouridine synthase" amino acids 374 to 532 (159 residues), 154.6 bits, see alignment E=9.3e-50

Best Hits

KEGG orthology group: K06178, ribosomal large subunit pseudouridine synthase B [EC: 5.4.99.12] (inferred from 45% identity to bth:BT_3872)

Predicted SEED Role

"Ribosomal large subunit pseudouridine synthase B (EC 4.2.1.70)" in subsystem Two cell division clusters relating to chromosome partitioning (EC 4.2.1.70)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9YWE9 at UniProt or InterPro

Protein Sequence (542 amino acids)

>CA264_17510 pseudouridylate synthase (Pontibacter actiniarum KMM 6156, DSM 19842)
MAKFNERPDRDNAGTGRRGADRDRSDRPSNDREGKKIFNRRDKYERPAEGGEGRERRSFG
DRDNRRDNDRKPFNSDRREGGDRRSFNSDRREGGERRSYGGDRAERREGGERRSFNSDRR
EGGERRSYGNDRREGGERRSFGGDRREGGERGERRSFNTDRPERRDNDRRSFGADRRDDR
RGGEREGGERRSFGGDRREGGDRRSFSSDRPRREDGDRRSYGSDRRDDRRGGDNDRRFSR
EGKPSFGEKRGFREDRGERGGKSFGAKGRSGDRFRGNEEEPKTPNYNLSRYKDNPRIKKS
NREEESDGTVRLNRYIANAGVCSRREADVLIESGEIKVNGEVITEMGYKVQPTDNVYYGK
KLLSREKMVYVLLNKPKDFLTTTDDPEGRKTVMSLVANASKERIFPVGRLDRNTTGLLLF
TNDGEVAQKLTHPSNNIKKIYQVELDKPITKADYVKVAEGVELEDGKAEVDDVAILGESN
KFLGLEIHIGRNRIVRRIFEHLGYDVVSLDRVQYAGLTKKDVPRGEWRYLTEKEVIQLKY
FM