Protein Info for CA264_16785 in Pontibacter actiniarum KMM 6156, DSM 19842

Annotation: methionine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1237 TIGR02082: methionine synthase" amino acids 11 to 1194 (1184 residues), 1767.4 bits, see alignment E=0 PF02574: S-methyl_trans" amino acids 16 to 323 (308 residues), 328.6 bits, see alignment E=1.2e-101 PF00809: Pterin_bind" amino acids 359 to 597 (239 residues), 212 bits, see alignment E=2.8e-66 PF02607: B12-binding_2" amino acids 656 to 729 (74 residues), 85.1 bits, see alignment 7.7e-28 PF02310: B12-binding" amino acids 749 to 838 (90 residues), 66.7 bits, see alignment 4.5e-22 PF02965: Met_synt_B12" amino acids 939 to 1211 (273 residues), 422 bits, see alignment E=2.7e-130

Best Hits

Swiss-Prot: 62% identical to METH_PSEAE: Methionine synthase (metH) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00548, 5-methyltetrahydrofolate--homocysteine methyltransferase [EC: 2.1.1.13] (inferred from 62% identity to avn:Avin_29240)

Predicted SEED Role

"5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13)" in subsystem Methionine Biosynthesis (EC 2.1.1.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9YVQ9 at UniProt or InterPro

Protein Sequence (1237 amino acids)

>CA264_16785 methionine synthase (Pontibacter actiniarum KMM 6156, DSM 19842)
MTKLHPIHKLLQERVLVLDGAMGTMIQRYQLQEADFRGERFQDHASDLKGNNDLLSITRP
DVIKAIHAEYLEAGADIIETNTFSGTSIAMADYHLEHIVYELNYESARLAREAADEIEAK
VPGKPRFVAGAIGPTNRTASLSPDVNNPGYRAITFDQLVEAYCEQVRGLVDGGSNLLLVE
TVFDTLNCKAALFAIDQYVQDGGKALPIMVSGTITDASGRTLSGQTVEAFWNSISHAPIL
SIGFNCALGARQLKTHIKELSRISDCYISAYPNAGLPNAFGGYDETAQQMGEIVEEYLKE
GLVNILGGCCGTTPVHTKVISDLVQKYKPHVPTPVAPLPRYSGLEPLTIYPESLFVNVGE
RTNVTGSKKFARLIVEEKFEEALSVAQQQVEGGAQIIDVNMDEGMLDSEQAMTNFLNLIA
SEPDISKLPIMIDSSKWSVIEAGLKCVQGKSIVNSISLKEGEENFKKVARKVRQYGAAVV
VMAFDEQGQADTFERRIEICEKSYRILVDEVGFPPQDIIFDPNILAIATGIEEHNNYAVD
YIETIKWIKANLPHALVSGGVSNLSFSFRGNDVVREAMHTVFLYYAVQAGMDMGIVNAGM
MGVYSEIPTELRDLIEDVIFNRHPDATEKLVTYAETIKGKGKGAAQADNSWREAPVEERL
KHALVKGIVEFIEEDTEEARQKATKTLEVIEGPLMAGMTVVGDLFGAGKMFLPQVVKSAR
VMKKSVAYLLPFMEAEKLASDTSKSTAGTILMATVKGDVHDIGKNIVGVVLACNNYEVID
LGVMVSLDKILAEAEAQQVDIIGLSGLITPSLDEMVYVAQEMERRGMKIPLLIGGATTSR
VHTAVKIAPQYSGPVVHVHDASRSVTVVSSLLGSDRENYIAEIKAEYEKLREDHLNRTKD
RAFASIGEARANKYKVDWAATRPTKPSFLGNKVYTNYPLSEIVPFIDWTPFFHTWELKRQ
YPKILDDAELGTEARKLFADAQQMLQEIVDNQLLEARAVVGFYPANVEADDTIEVYADDS
RENELTEFHTLRQQGKKGQGVPNMAFSDFIAPKETGVQDYIGGFVVSAGFGIEKMLEQYE
AEHDDYRSIMAKALADRLAEAFAELMHLKVRRELWGYSPEEQLSNEDLIKEQYKGIRPAP
GYPGCPDHTEKITLFNLLNAEEQTGIILTENLAMYPASSVSGLYFSHPESKYFGLGKIGE
DQVADLAQRKNMPQEELERWLSPNLNYEPKPLAQQTA