Protein Info for CA264_15700 in Pontibacter actiniarum KMM 6156, DSM 19842

Annotation: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01225: Mur_ligase
  • amino acids 15 to 85 (71 residues), 27.4 bits, E=5.3e-10
• TIGR01143: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase
  • amino acids 20 to 426 (407 residues), 387.8 bits, E=3.2e-120
• PF08245: Mur_ligase_M
  • amino acids 96 to 276 (181 residues), 134.1 bits, E=9.8e-43
• PF02875: Mur_ligase_C
  • amino acids 299 to 376 (78 residues), 41.5 bits, E=2e-14

Best Hits

KEGG orthology group: K01929, UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanine ligase [EC: 6.3.2.10] (inferred from 51% identity to aas:Aasi_1231)

Predicted SEED Role

"UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanine ligase (EC 6.3.2.10)" in subsystem Methicillin resistance in Staphylococci or Peptidoglycan Biosynthesis (EC 6.3.2.10)

MetaCyc Pathways

• peptidoglycan biosynthesis I (meso-diaminopimelate containing) (11/12 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (7/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-diaminopimelate containing) (6/8 steps found)
• peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
• peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
• peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
• peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)

KEGG Metabolic Maps

• Lysine biosynthesis
• Peptidoglycan biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 6.3.2.10

Use Curated BLAST to search for 6.3.2.10

Sequence Analysis Tools

See A0A1X9YV41 at UniProt or InterPro

Protein Sequence (429 amino acids)

>CA264_15700 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase (Pontibacter actiniarum KMM 6156, DSM 19842)
MQNSIEFLYQRYLECHHVSTDSRAEQNQSMFFALNGPNFKGAKFAQMALEKGARYAVVDD
PGMAADNVFVVEDTLLALQELARHHRRQLSIPVIGITGSNGKTTSKELVHAVLSQKYNVL
YTQGNLNNHIGVPLTLLRITPEHEMAIIEMGANHIGEIAQLSAIALPTHGLITNIGKAHL
EGFGSLEGVARGKSELYLHLDQHGGTVFINTGNEHLMRMARRIENKITYPAPHDYYHSEL
LEASPYVVYRDEEGHVVHTQLVGSYNYENMAAAACIGKFFGVPLQQANEAIARYSPVNNR
SQVLQQGSNTIILDAYNANPTSMAAAVRNFGNMKAQHKVVILGDMFEMGAESEAEHRLLG
EVVAEQPFDTVILCGKHMKYAAEVSEDFLYFETKPELQTWLRKNPIAESYVLIKGSRGMG
LETLTAELG