Protein Info for CA264_15430 in Pontibacter actiniarum KMM 6156, DSM 19842

Annotation: carbamoyl phosphate synthase large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01369: carbamoyl-phosphate synthase, large subunit
  • amino acids 2 to 929 (928 residues), 1317.7 bits, E=0
• PF02786: CPSase_L_D2
  • amino acids 128 to 334 (207 residues), 216.5 bits, E=1.1e-67
  • amino acids 670 to 869 (200 residues), 91.2 bits, E=2.5e-29
• PF02787: CPSase_L_D3
  • amino acids 425 to 502 (78 residues), 85.2 bits, E=8.6e-28
• PF07478: Dala_Dala_lig_C
  • amino acids 677 to 840 (164 residues), 24.2 bits, E=7.9e-09
• PF02222: ATP-grasp
  • amino acids 679 to 842 (164 residues), 32.2 bits, E=2.9e-11

Best Hits

Swiss-Prot: 56% identical to CARB_ARCFU: Carbamoyl-phosphate synthase large chain (carB) from Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)

KEGG orthology group: K01955, carbamoyl-phosphate synthase large subunit [EC: 6.3.5.5] (inferred from 82% identity to chu:CHU_3278)

Predicted SEED Role

"Carbamoyl-phosphate synthase large chain (EC 6.3.5.5)" in subsystem De Novo Pyrimidine Synthesis or Macromolecular synthesis operon (EC 6.3.5.5)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (42/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (17/18 steps found)
• UMP biosynthesis II (6/6 steps found)
• L-arginine biosynthesis I (via L-ornithine) (8/9 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (8/9 steps found)
• L-citrulline biosynthesis (7/8 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• L-glutamate and L-glutamine biosynthesis (6/7 steps found)
• L-citrulline degradation (3/3 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-arginine biosynthesis II (acetyl cycle) (8/10 steps found)
• superpathway of arginine and polyamine biosynthesis (13/17 steps found)
• UMP biosynthesis I (5/6 steps found)
• UMP biosynthesis III (5/6 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (7/9 steps found)
• superpathway of L-citrulline metabolism (9/12 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-arginine degradation V (arginine deiminase pathway) (3/4 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (3/4 steps found)
• L-arginine biosynthesis IV (archaea) (4/9 steps found)
• allantoin degradation IV (anaerobic) (2/9 steps found)
• guadinomine B biosynthesis (2/13 steps found)

KEGG Metabolic Maps

• Glutamate metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.5

Use Curated BLAST to search for 6.3.5.5

Sequence Analysis Tools

See A0A1X9YUZ0 at UniProt or InterPro

Protein Sequence (937 amino acids)

>CA264_15430 carbamoyl phosphate synthase large subunit (Pontibacter actiniarum KMM 6156, DSM 19842)
MPKDTSIKSVLIIGSGPIIIGQACEFDYSGSQAARSLREEGIEVTLINSNPATIMTDPVT
ADNVYLKPLEKKYIIEILEKHKIDAVLPTMGGQTALNLAIDCEKAGIWQKYGVRIIGVDI
KAIETTEDREEFRLKMLELGVNVCKGETATSFLEGKEIAQEIGFPLVIRPSFTLGGYGGG
FVHTPEEFDTALTRGLHASPTHEVLIEQSILGWKEYELELLRDNMGNIIIICSIENFDPM
GVHTGDSITVAPAMTLPDTVYQQMRDLAIKMMNGIGQFAGGCNVQFSVNPEDDTIIAIEI
NPRVSRSSALASKATGYPIAKIAAKLAIGYNLDELKNAITKTTSAFFEPALDYVIVKIPR
WNFDKFPGVNRTLGLQMKSVGEVMGIGRTFQEALQKACQSLEIKRNGIGADGKEKTNYDE
LIHGLSNPSWNRLFNIKDAMRIGIPTSTIQKLTKIDPWFLAQIEELDMMEKEIEKYSLGT
IPAELLREAKVKGYADRQIAHLLRCKESEVHSVRTELGIKRVFKMVDTCAAEFEANTPYY
YSTFEGENESIVSDKKKVVVLGSGPNRIGQGIEFDYSCVHGVLAARECGYETIMINCNPE
TVSTDFDISDKLYFEPVFWEHIYDIILHEKPEGVIVQLGGQTALKLAEKLDRFGIKVLGT
SYQALDLAEDRGSFSSLLRDLAIPYPPFAVIETAEEALELSKELKFPLLVRPSYVLGGQN
MKIVINEKELEAHVIDLLKDHPGNKVLLDHFLDNAIEAEADAICDGEDVYICGIMEHIEP
AGIHSGDSYAVLPPFDLSENVMRQIEEHTKKIAVALNTVGVINIQFAIKNEVVYIIEANP
RASRTFPFIAKAYREPYINYATKIMLGAKKVKDFTFNPYKHGYAIKVPVFSYNKFPEVNK
ELGPEMKSTGEAIYFIDDLQDDYFTKVYSERNLYLSK