Protein Info for CA264_15355 in Pontibacter actiniarum KMM 6156, DSM 19842
Annotation: bifunctional UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine ligase/alanine racemase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K01775, alanine racemase [EC: 5.1.1.1] (inferred from 48% identity to bhl:Bache_1322)Predicted SEED Role
"UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanine ligase (EC 6.3.2.10) / Alanine racemase (EC 5.1.1.1)" in subsystem Alanine biosynthesis or Pyruvate Alanine Serine Interconversions or Methicillin resistance in Staphylococci or Peptidoglycan Biosynthesis (EC 5.1.1.1, EC 6.3.2.10)
MetaCyc Pathways
- peptidoglycan biosynthesis I (meso-diaminopimelate containing) (11/12 steps found)
- UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
- UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (7/8 steps found)
- alanine racemization (1/1 steps found)
- UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-diaminopimelate containing) (6/8 steps found)
- peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
- anaerobic energy metabolism (invertebrates, cytosol) (5/7 steps found)
- peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
- peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
- L-alanine degradation I (1/2 steps found)
- peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)
- superpathway of anaerobic energy metabolism (invertebrates) (11/17 steps found)
- ansatrienin biosynthesis (1/9 steps found)
- cyclosporin A biosynthesis (1/15 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 5.1.1.1, 6.3.2.10
Use Curated BLAST to search for 5.1.1.1 or 6.3.2.10
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A1X9YV06 at UniProt or InterPro
Protein Sequence (818 amino acids)
>CA264_15355 bifunctional UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine ligase/alanine racemase (Pontibacter actiniarum KMM 6156, DSM 19842) MLTFQQLQSIVQGREVQFVQPLPVVHLLTDSRKLSQPAGTVFFAVRGKYNDGHQYLPQLY AQGVRAFVVEQGEPGELATRYPEANILLVESSLEALQQLAAWHRSQFSIPVIGVTGSNGK TIVKEWLSQLLSPDELVVKSPRSYNSQLGVPLSVWQLQPNHTLAIFEAGISQPGEMERLQ QVIQPTLGVFTNVGSAHDEGFASREQKIAEKLKLFQGVELLFYCADYELLHQAVQAQGIK SFTWSRHQPEANLFINATTTAGQKTIVVYTYQGEKQRLAIPFTDEASVENALHCLALLLY RRLPLSEVQDRLDRLHPVAMRLEMKEAINGCYLIDDTYNNDLAGLAIALDLQASQPQRGR RTLILSDVLESGLPEEQLYEKVAGLAQAHKVQRLIGIGPTISKYAHLFPQGSFYTSTADF LQAFDASSFRGELILVKGARVFGFEKIVQAFQQKVHGTVLEVNLDALVHNLNYYRSRVAP ATKLMVMVKAFAYGSGSFEVANLLQFHRVDYLAVAYVDEGVALRENGITLPIMVMNPSQD SFAKLRQYNLEPEIYSVEQLQDFVSSLEPEATYKIHLKLDTGMHRLGFVPEDFEALFELL HRYPQVQVASTFSHLAGADEAVHNDFSQLQVSRFRSMAAAVEERLKYKVIKHILNSAGIV RFPEHQLDMVRLGIGLYGVEATGSEQEALRPVSTLKTTVSQVKSIKQGETVGYSRKGIAS SDRTTATIAIGYADGYDRRFSNGVGQVLINGHRCPLIGNVCMDMCMVDVTGVAVKAGDVA IVFGPQLTLVELAQSIGTIPYELLTNVSTRVKRVFYAE