Protein Info for CA264_15280 in Pontibacter actiniarum KMM 6156, DSM 19842

Annotation: tRNA 2-selenouridine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 342 PF00581: Rhodanese" amino acids 12 to 126 (115 residues), 44.8 bits, see alignment E=7.5e-16 TIGR03167: tRNA 2-selenouridine synthase" amino acids 17 to 314 (298 residues), 342.3 bits, see alignment E=1.5e-106

Best Hits

KEGG orthology group: K06917, tRNA 2-selenouridine synthase [EC: 2.9.1.-] (inferred from 48% identity to sli:Slin_4991)

Predicted SEED Role

"Selenophosphate-dependent tRNA 2-selenouridine synthase" in subsystem Selenocysteine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.9.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9YUS8 at UniProt or InterPro

Protein Sequence (342 amino acids)

>CA264_15280 tRNA 2-selenouridine synthase (Pontibacter actiniarum KMM 6156, DSM 19842)
MVNTITIEEFMQKAAQLPVLDVRAPKEYEVGHIPAAHSFPIFDDKQRAVIGTAYKQQGHD
PAVLIGLDSFGPRMSGFVKEATKLAKDKELLVHCWRGGMRSGAMAWLLDFSGFKVHLLKG
GYKAYRHLMHAQFEKPRKLVILGGLTGTGKTDILPHLQQLGQQSIDLEGLANHKGSAFGS
IDMPPQPSTEHFENLLGMQLLQLSEQQALWLEDESKTIGRVVMPKPLHDQMQAAPTIVLQ
VPHKLRVQKLAQEYCHTNEAILAASILKIKKRLGGLATKEALAAIAEGDMEKMVEIALVY
YDKAYNFDLAKRQHLIPLELQSINPEENAAQVLQLAQKHKLL