Protein Info for CA264_12585 in Pontibacter actiniarum KMM 6156, DSM 19842

Annotation: molecular chaperone DnaK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 642 TIGR02350: chaperone protein DnaK" amino acids 3 to 599 (597 residues), 999.6 bits, see alignment E=2.3e-305 PF00012: HSP70" amino acids 4 to 599 (596 residues), 892.1 bits, see alignment E=1.6e-272 PF06723: MreB_Mbl" amino acids 118 to 372 (255 residues), 56.1 bits, see alignment E=2.9e-19

Best Hits

Swiss-Prot: 80% identical to DNAK_CYTH3: Chaperone protein DnaK (dnaK) from Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469)

KEGG orthology group: K04043, molecular chaperone DnaK (inferred from 82% identity to mtt:Ftrac_1865)

MetaCyc: 65% identical to chaperone protein DnaK (Escherichia coli K-12 substr. MG1655)
Non-chaperonin molecular chaperone ATPase. [EC: 3.6.4.10, 5.6.1.7]

Predicted SEED Role

"Chaperone protein DnaK" in subsystem Heat shock dnaK gene cluster extended or Protein chaperones

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.4.10 or 5.6.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9YTL8 at UniProt or InterPro

Protein Sequence (642 amino acids)

>CA264_12585 molecular chaperone DnaK (Pontibacter actiniarum KMM 6156, DSM 19842)
MGKIIGIDLGTTNSCVAVMEGNEPVVIPNSEGRRTTPSIVAFLDNGNGERKVGDPAKRQA
ITNPQNTIASIKRFMGHSYNEVSEEAKQVSYKVQQGSNNTVRVQIGDREYTPQEISAMVL
QKMKATAEEYLGTSVTEAVITVPAYFNDAQRQATKEAGQIAGLEVKRIINEPTAAALAYG
LDKKSVDQKIAVFDLGGGTFDISILELGDGVFEVLSTNGDTHLGGDDFDQVIINWLAEEF
KKDEGLDLRKDPMALQRLKEAAEKAKIELSSSQETEINLPYIMPVDGVPKHLVRKLTRAK
FEQLSDDLIRRSMEPCKKALQDAGLSTSDIDEVILVGGSTRIPKVQEEVEKFFGKKPSKG
VNPDEVVAIGAAIQGGVLTGEVKDVLLLDVTPLSLGIETMGGVFTKLIESNTTIPTKKSE
TFSTASDNQPSVEIHVLQGERPMAKDNRTIGRFHLSDIPPAPRGVPQIEVIFDIDANGIL
HVTAKDKATGKEQKIRIEASSGLSEQEIEKMRKEAEANAEADKKAKEEIEKLNTADSMIF
QTEKQLKEYGDKLSAGNKSNIENALAELKKAHEAKDVAGIDSAIENLNNAWSAASQEMYA
ATQGQGAPGAEAGGNGQAGGAQGAANDGGVTDVDYEEVDGNK