Protein Info for CA264_12365 in Pontibacter actiniarum KMM 6156, DSM 19842

Annotation: asparagine synthetase B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01536: asparagine synthase (glutamine-hydrolyzing)
  • amino acids 2 to 540 (539 residues), 382.8 bits, E=1.7e-118
• PF13522: GATase_6
  • amino acids 29 to 152 (124 residues), 115.3 bits, E=4e-37
• PF13537: GATase_7
  • amino acids 44 to 156 (113 residues), 121.1 bits, E=5.6e-39
• PF12481: DUF3700
  • amino acids 66 to 156 (91 residues), 36 bits, E=1.1e-12
• PF00733: Asn_synthase
  • amino acids 234 to 605 (372 residues), 266.5 bits, E=1.1e-82

Predicted SEED Role

"Asparagine synthetase [glutamine-hydrolyzing] (EC 6.3.5.4)" in subsystem Cyanophycin Metabolism or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 6.3.5.4)

MetaCyc Pathways

• L-citrulline biosynthesis (7/8 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• L-glutamate and L-glutamine biosynthesis (6/7 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• superpathway of L-citrulline metabolism (9/12 steps found)
• L-asparagine biosynthesis I (1/1 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (3/4 steps found)
• superpathway of L-asparagine biosynthesis (1/2 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Nitrogen metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.5.4

Sequence Analysis Tools

See A0A1X9YTL3 at UniProt or InterPro

Protein Sequence (611 amino acids)

>CA264_12365 asparagine synthetase B (Pontibacter actiniarum KMM 6156, DSM 19842)
MCGIFGKVGSLRERDMHVATEMLCHRGPDGQVHKTYAQNVHFFHARLSIVDIEGGTQPMD
DEGLAIIFNGEIYNHQELRTKFGLTGKTRSDTETVLLMYKQLGLDMLSHMDGMFAIALFD
KKQGKVFLLRDRAGKKPLYIGVADDGIYFGSEQNLLQKIMRPSIYLPSIAEYLQTGMVYG
KNTPYKDIYELQPGHRISIDVENLSMSEQQQWWSIEPYYEQVLELQEQEALALLDEKLKI
AVKRRVESSDLEVGCFLSGGIDSGIMAAMASELKPNLRTFTVKFQNEYDESVVAKQVAKY
LNTNHQELEVSYDKLTNDFESIVRAYGEPFMDDSQIPSYYVAREAKKHVTVIINGDGGDE
LFGGYRRYVPFAHSFFRNGVVKGASKAVAPFLPAPASKMNMYNYLYRLVKLSSRNDFEQY
LSASTDVLFDYKRDYKVQPEPSFQTLVARNFSKNISGLRKIMLTDFQGILPYILLKKIDI
SSMQSSLEGRSPFLSKEIMEFAPSLSDNLKIRGGTTKYLLRKLASQYLPAGNDKLPKKGF
EVPIINLVDKDINPLLKDYVNSSDCLYKEVIDPKLVQRLMNGTLNISKDKRAKILFSLLT
MEIWYKNQKQV