Protein Info for CA264_11055 in Pontibacter actiniarum KMM 6156, DSM 19842

Annotation: cysteine desulfurase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02006: cysteine desulfurase IscS
  • amino acids 2 to 404 (403 residues), 656.6 bits, E=7.4e-202
• PF00266: Aminotran_5
  • amino acids 6 to 367 (362 residues), 302.2 bits, E=1e-93
• PF00155: Aminotran_1_2
  • amino acids 53 to 289 (237 residues), 36.4 bits, E=7.2e-13
• PF01212: Beta_elim_lyase
  • amino acids 54 to 272 (219 residues), 38.6 bits, E=1.6e-13
• PF01041: DegT_DnrJ_EryC1
  • amino acids 69 to 186 (118 residues), 38.7 bits, E=1.5e-13

Best Hits

Swiss-Prot: 63% identical to ISCS_PHOLL: Cysteine desulfurase IscS (iscS) from Photorhabdus luminescens subsp. laumondii (strain DSM 15139 / CIP 105565 / TT01)

KEGG orthology group: K04487, cysteine desulfurase [EC: 2.8.1.7] (inferred from 80% identity to phe:Phep_1563)

MetaCyc: 63% identical to cysteine desulfurase IscS (Escherichia coli K-12 substr. MG1655)
Cysteine desulfurase. [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]

Predicted SEED Role

"Cysteine desulfurase (EC 2.8.1.7), IscS subfamily" in subsystem Alanine biosynthesis or Thiamin biosynthesis (EC 2.8.1.7)

MetaCyc Pathways

• superpathway of thiamine diphosphate biosynthesis I (9/10 steps found)
• superpathway of L-alanine biosynthesis (4/4 steps found)
• superpathway of thiamine diphosphate biosynthesis II (9/11 steps found)
• thiazole component of thiamine diphosphate biosynthesis I (5/6 steps found)
• L-alanine biosynthesis III (1/1 steps found)
• L-cysteine degradation IV (1/1 steps found)
• thiazole component of thiamine diphosphate biosynthesis II (5/7 steps found)
• cytidylyl molybdenum cofactor sulfurylation (1/2 steps found)
• bis(guanylyl molybdopterin) cofactor sulfurylation (1/3 steps found)
• tRNA-uridine 2-thiolation (mammalian mitochondria) (1/4 steps found)
• tRNA-uridine 2-thiolation (yeast mitochondria) (1/4 steps found)
• molybdopterin biosynthesis (2/6 steps found)
• tRNA-uridine 2-thiolation (thermophilic bacteria) (1/5 steps found)
• [2Fe-2S] iron-sulfur cluster biosynthesis (3/10 steps found)
• tRNA-uridine 2-thiolation (cytoplasmic) (1/8 steps found)
• tRNA-uridine 2-thiolation and selenation (bacteria) (2/11 steps found)

KEGG Metabolic Maps

• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.8.1.7

Use Curated BLAST to search for 2.8.1.7

Sequence Analysis Tools

See A0A1X9YSR6 at UniProt or InterPro

Protein Sequence (404 amino acids)

>CA264_11055 cysteine desulfurase (Pontibacter actiniarum KMM 6156, DSM 19842)
MLTLPIYLDNNATTPLDPRVLEAMMPYMTNMFGNAASRNHAFGWQAEEAVDYARDQIASL
INCSPKEIIFTSGATESDNLAIKGVFEMYASKGNHIITATTEHKAVLDTCKHIEKIGGKV
TYLQVNDKGLIDLKELEEAITDKTILISIMFANNEIGVIQPVREISAIAKKHGILFFTDA
TQAVGKIPVDVEADGIDLMAFSAHKMYGPKGVGALYVRRKNPRVKVTAQMDGGGHERGMR
SGTLNVPGIVGLGKAAEIAKQDMESDTKRIAAMRDRLERELTTIEESYINGSTEHRLPHV
SNISFKYVEGEGLMMGVKDLAVSSGSACTSASLEPSYVLKALGLSDDLAHSSLRFGLSRF
TTDEEIDFAIGHVKEAVAKLRDLSPLWEMFKEGIDLNSIEWAEH