Protein Info for CA264_10825 in Pontibacter actiniarum KMM 6156, DSM 19842

Annotation: UDP-N-acetylmuramate--L-alanine ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01082: UDP-N-acetylmuramate--L-alanine ligase
  • amino acids 9 to 455 (447 residues), 381.7 bits, E=2.9e-118
• PF01225: Mur_ligase
  • amino acids 9 to 113 (105 residues), 53.6 bits, E=3.5e-18
• PF08245: Mur_ligase_M
  • amino acids 118 to 299 (182 residues), 87.6 bits, E=1.7e-28
• PF02875: Mur_ligase_C
  • amino acids 320 to 404 (85 residues), 63.8 bits, E=2.3e-21

Best Hits

Swiss-Prot: 46% identical to MURC_BACV8: UDP-N-acetylmuramate--L-alanine ligase (murC) from Bacteroides vulgatus (strain ATCC 8482 / DSM 1447 / JCM 5826 / NBRC 14291 / NCTC 11154)

KEGG orthology group: K01924, UDP-N-acetylmuramate--alanine ligase [EC: 6.3.2.8] (inferred from 52% identity to sli:Slin_4753)

Predicted SEED Role

"UDP-N-acetylmuramate--alanine ligase (EC 6.3.2.8)" in subsystem Peptidoglycan Biosynthesis (EC 6.3.2.8)

MetaCyc Pathways

• peptidoglycan biosynthesis I (meso-diaminopimelate containing) (11/12 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (7/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-diaminopimelate containing) (6/8 steps found)
• peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
• peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
• peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
• peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)

KEGG Metabolic Maps

• D-Glutamine and D-glutamate metabolism
• Peptidoglycan biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.8

Sequence Analysis Tools

See A0A1X9YSM0 at UniProt or InterPro

Protein Sequence (469 amino acids)

>CA264_10825 UDP-N-acetylmuramate--L-alanine ligase (Pontibacter actiniarum KMM 6156, DSM 19842)
MRLENYSYIYFLGIGGIGMSAIARWFKAKGYPVWGYDKTRTSLTEALEGEGIAVHYEDDV
AQLPEEILRHQEKTLVVLTPAIPAEHTEWAYLREQGYAIKKRSEVLGIITASAYTVAVAG
THGKTTTSSIVAHLLHHAGVDCSAFLGGIATNLNSNLLIGKGEADREVVVVEADEYDRSF
LTLFPDVAIVTSADPDHLDIYGDKEELIRTFQRFISQIKPGGHLFLHEATDPRLTAQVQE
GVRVYKYSLDSGDAYVQQLNINGRWFEFDAVSPFGDLQGLRLGVPGYHNTENTLAAVLTA
QVLQVPAQSIKSGVETFAGVKRRFEFVFEGNGKVYIDDYAHHPKEIDAFMGSLRALYPNK
RIKVIFQPHLFSRTRDFADAFAESLSKADELVLLDIYPAREKPMPGVTSDMILKQVTSPV
KELMSKEEVVGNLLKNGDFDVLATLGAGDIDTLVKPLKNILENKGYGLE