Protein Info for CA264_10100 in Pontibacter actiniarum KMM 6156, DSM 19842

Annotation: nitrite reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 738 PF04879: Molybdop_Fe4S4" amino acids 35 to 79 (45 residues), 50.4 bits, see alignment 2.6e-17 PF00384: Molybdopterin" amino acids 91 to 516 (426 residues), 192.6 bits, see alignment E=1.5e-60 PF01568: Molydop_binding" amino acids 610 to 716 (107 residues), 101.1 bits, see alignment E=5.5e-33

Best Hits

KEGG orthology group: K00372, nitrate reductase catalytic subunit [EC: 1.7.99.4] (inferred from 75% identity to shg:Sph21_2694)

Predicted SEED Role

"Assimilatory nitrate reductase large subunit (EC:1.7.99.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.99.4)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.7.99.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9YSB1 at UniProt or InterPro

Protein Sequence (738 amino acids)

>CA264_10100 nitrite reductase (Pontibacter actiniarum KMM 6156, DSM 19842)
MAKLPITVDKIIEDFGPHEAYAPKQGFQGHAEPDKLVKTHCCFCGMQCGIQLKVKDDVVV
GFEPWMEFPFNEGRMCPKGVVRYLQNNHPDRLLSPIERVEGEGFKPIDWETAMSRTVSEI
KRIQSTYGNDAFAMLSGVSLTNELSYMVGKFARVALKTANIDYNGRLCMVSAGAGNKKAF
GRDRASNSWADLKHAEVIMICGSNVSETFPTLIHYIWEARDNGAKLIVVDPRITPVARTA
DLYLPVRPGTDSALFGAMLNYLVENDLLDHDFIDNYTSGFEETISSVKDHTLEWAEGITG
IPAASIRQAAEMWGRAKTSFMLHARGIEQHSKGVDNVLGCINLVLATGRIGKPYCGYNTI
TGQGNGQGGREHGHKCDQLPGNRDITNPEHRKYIASVWGIDEKDMPGKGLSAYELVEAIH
RGEIKGLISICFNPIVSLPNNNMVREALEKLEFYVAIDFFLNETARHANIVMAGALHEEE
EGTGTTAEGRVVKINAAVPPPGDARADAAILKELAARLGVGDKFAYRNSEDIFNELRVAS
KGSSVDYYGITYEKIEKNFGVFWPCPTLDHPGTPRLWEDKKFLTPDGKAHFNPAPYRLSA
EPVDAEYPILLTTGRVVSQYLSGTQTRRLGRLNDSYPEPLLEIHPSLAQQYGIKEREIVK
VMTRRGEAEFPAQIVTTIRQDTVFIPYHWGGRKSANQLTIGVLDPTSKMPEFKVCACKLV
PTGVIAPPSKESLAYQSR