Protein Info for CA264_08570 in Pontibacter actiniarum KMM 6156, DSM 19842

Annotation: glycogen debranching enzyme

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 710 TIGR02100: glycogen debranching enzyme GlgX" amino acids 8 to 704 (697 residues), 1079.1 bits, see alignment E=0 PF02922: CBM_48" amino acids 12 to 101 (90 residues), 74.2 bits, see alignment E=1.3e-24 PF00128: Alpha-amylase" amino acids 196 to 284 (89 residues), 40.6 bits, see alignment E=3.7e-14

Best Hits

Swiss-Prot: 61% identical to GLGX_MYCTO: Glycogen operon protein GlgX homolog (glgX) from Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)

KEGG orthology group: K02438, glycogen operon protein GlgX [EC: 3.2.1.-] (inferred from 75% identity to cpi:Cpin_2576)

MetaCyc: 58% identical to isoamylase (Sulfolobus acidocaldarius)
Isoamylase. [EC: 3.2.1.68]

Predicted SEED Role

"Glycogen debranching enzyme (EC 3.2.1.-)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 3.2.1.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.-

Use Curated BLAST to search for 3.2.1.- or 3.2.1.68

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9YRJ9 at UniProt or InterPro

Protein Sequence (710 amino acids)

>CA264_08570 glycogen debranching enzyme (Pontibacter actiniarum KMM 6156, DSM 19842)
MDIENYPGSPYPLGATWDGEGVNFALFSENATAVELCLFNSEDDKTEAAKVKMTERTHQV
WHTYLPGVKPGQLYGFRVHGPFEPHNGHRFNPHKLLLDPYAKAISGTIEWHDALFGYTMG
DPQKDLSFSKTDSAQYIPKTVVIDPSFDWGNDRKPKIPYHKTIIYEAHVKGFTKLHPEIP
EEIRGTYAGLAHPVTIKYLKQLGITAIELMPVHHFITDRHLADKNLANYWGYNTIGFFAP
DVRYSSSGTHGEQVVEFKQMVKALHQAGIEVILDVVYNHTAEGNHMGPTLSFRGIDNAAY
YRLTEDQRYYMDYTGTGNTLNANLPNVLRLIMDSLRYWILEMHVDGFRFDLAATLARELH
EVDRLSAFFDIIHQDPIISQVKLIAEPWDVGEGGYHVGNFPPGWAEWNGKYRDCIRDYWR
GADSMLAEFAQRFTGSSDLYKDDYRSPTASINFVTAHDGFTLHDLVSYNEKHNEANGEGN
NDGESHNRSWNCGVEGPTKDAEVVELRNRQIRNFMTTLFLSQGVPMLVSGDELGRTQGGN
NNAYCQDNEISWIDWGNVDQDLLSFTRRLISFCKKHPVFNRRRWFQGKPIKGKVKDIAWF
LPEGKEMTEENWNHDFAKSLAVFLSGMGIHSKGPKGEQVQDDSFYVIFNAHYEPLKYKLP
YVKYGKSWTKVLDTAAPDSLDEEEEVYLPNETVEVAARSVVVLHHQEEKR