Protein Info for CA264_07400 in Pontibacter actiniarum KMM 6156, DSM 19842

Annotation: threonine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 645 PF02824: TGS" amino acids 2 to 61 (60 residues), 56.4 bits, see alignment 4.8e-19 TIGR00418: threonine--tRNA ligase" amino acids 72 to 634 (563 residues), 746.6 bits, see alignment E=1.1e-228 PF07973: tRNA_SAD" amino acids 169 to 218 (50 residues), 59.9 bits, see alignment 3.8e-20 PF00587: tRNA-synt_2b" amino acids 325 to 535 (211 residues), 166.4 bits, see alignment E=1.4e-52 PF03129: HGTP_anticodon" amino acids 548 to 636 (89 residues), 85 bits, see alignment E=6.8e-28

Best Hits

Swiss-Prot: 79% identical to SYT_CYTH3: Threonine--tRNA ligase (thrS) from Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469)

KEGG orthology group: K01868, threonyl-tRNA synthetase [EC: 6.1.1.3] (inferred from 79% identity to chu:CHU_1643)

MetaCyc: 44% identical to threonine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Threonine--tRNA ligase. [EC: 6.1.1.3]; 6.1.1.3 [EC: 6.1.1.3]; 3.1.1.- [EC: 6.1.1.3]

Predicted SEED Role

"Threonyl-tRNA synthetase (EC 6.1.1.3)" (EC 6.1.1.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9YQY9 at UniProt or InterPro

Protein Sequence (645 amino acids)

>CA264_07400 threonine--tRNA ligase (Pontibacter actiniarum KMM 6156, DSM 19842)
MINITLPDGSVRQYESGVTSLDIAQSISEGLARNVLAAKVNGAVWDATRAINQDATVQLL
TWNDEEGKNTFWHSSAHLLAEALEDLYPDVKFGIGPPVENGFYYDVDLGEHHLSQEDIAK
LEQKMLELARQKNVYLRSEVSKADAVDYFTKKGDQYKLDLIKDLEDGSITFYEQGKFVDL
CRGPHIPNTGFVKAAKITNIAGAYWRGDEKNKQLTRIYGITFPKQKELTEYLERVEEAKK
RDHRKLGKEMELFAFSEKVGMGLPLWLPKGTLLRERLEQFMRKAQMRAGYQPVVTPHIAS
KELYVTSGHYEKYGADSFQPIKTPNEGEEFLLKPMNCPHHCEIYKTRPRSYKELPVRFAE
FGTVYRYEQSGELHGLTRVRGFTQDDAHIFCRPDQVKEEFLKVIDLVLYVFKALGFEDYT
AQISLRDPENKAKYIGTDEAWEKAERAIIEASAEKGLSTVTELGEAAFYGPKLDFMVKDA
LGRKWQLGTIQVDYQLPERFQLEYIGADNQKHRPVMIHRAPFGSLERFVAVLIEHCGGNF
PLWLSPEQIAILPISEKYHDYAQQVYDRLQQDDIRGYVDNRDEKIGRKIRDAEVRKVPYM
IIVGEKEQENGAVSVRKHGEGDLGAMTVEEFSTFFQGKVAEMLNN