Protein Info for CA264_07285 in Pontibacter actiniarum KMM 6156, DSM 19842

Annotation: alpha-amlyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 618 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF09087: Cyc-maltodext_N" amino acids 28 to 114 (87 residues), 106.7 bits, see alignment E=9.5e-35 PF00128: Alpha-amylase" amino acids 165 to 483 (319 residues), 190.3 bits, see alignment E=1.1e-59 PF10438: Cyc-maltodext_C" amino acids 540 to 616 (77 residues), 85.6 bits, see alignment E=2.9e-28

Best Hits

KEGG orthology group: None (inferred from 53% identity to cao:Celal_2535)

Predicted SEED Role

"Neopullulanase (EC 3.2.1.135)" in subsystem Maltose and Maltodextrin Utilization (EC 3.2.1.135)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.135

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9YQU6 at UniProt or InterPro

Protein Sequence (618 amino acids)

>CA264_07285 alpha-amlyase (Pontibacter actiniarum KMM 6156, DSM 19842)
MHCKKLLLLFFLLSFITSTAFAQQEKLRVEPSFWWTDMKNPDLQLMLHGQNISHLQPELK
HPGVKVKAVTKVKNPNYLFVTLNIENAKPGKSDLKLKRDGKTVERYTYELRKRDPKSATR
QGFDTSDVLYLITPDRFANGNPKNDSVKGMDDKLNRSDKGGRHGGDISGLIDHLDYVDEM
GFTALWVNPVLENDQPSYSYHGYSTTDFYKVDPRFGSNEEYLQLSSLAKERGIKLVMDMI
LNHCGSAHWWMDDLPTDNWLNFQGDFKTTNHRREAIQDPHASAYDYRMHTEGWFVESMPD
LNQENELLANYLIQNTIWWIEYAGLGGIRMDTYSYPDPQFMSEWTHRVMEEYPNFNIVGE
EWSVNPAIVSYWQRGKQNWNGYVSYLPSLMDFPVQNALAESLKDDESSWESGWMKLYTML
VNDFLYPDPNNLVVFPDNHDMDRIYTQLNQDPELTKLAVTYVLTTRGIPQLYYGTEILMH
NDEPDDHGIIRTDFPGGWAGDKRNAFTGTGLTREEQDMHQFTKRLLNWRKNAEVIHTGKL
THYAPMKGVYVYFRDNGKERVMVVLNKNTEPYELELEKFSPQLSGLTQGTDVITGKTYDL
NQPTLALPQKASLVLELK